PA5-20478

antibody from Invitrogen Antibodies

Targeting: SLITRK5 bA364G4.2, KIAA0918, LRRC11

Western blot (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-20478

Provider

Invitrogen Antibodies

Product name

SLITRK5 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

A suggested positive control is NIH-3T3 cell lysate. PA5-20478 can be used with blocking peptide PEP-0598.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1 mg/mL

Storage

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

References

Submitted references

MUC21 controls melanoma progression via regulating SLITRK5 and hedgehog signaling pathway.

Liu X, Xiao Y, Xiong X, Qi X
Cell biology international 2022 Sep;46(9):1458-1467

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

2 Figure MUC21 suppressed SLITRK5 to regulate melanoma progression. (a) Heatmap of top 20 up/downregulated genes in MUC21 high expression patients in comparison with MUC21 low expression patients ( n = 462). (b) Western blotting of SLITRK5 in vector (VEC) or MUC21 overexpression (OE) A375/A875 cells. (c) Western blotting of SLITRK5 in vector (VEC) or SLITRK5 knockout (KO) A375/A875 cells. (d) Cell proliferation of vector (VEC) or SLITRK5 knockout (KO) A375/A875 cells. (e) Relative migrating cells of vector (VEC) or SLITRK5 knockout (KO) A375/A875 cells. (f) Immumohistochemical staining of SLITRK5 in melanoma patients divided into primary and metastatic groups ( n = 15 in each group). The scale bar was 100 mum. SLITRK5 expression was quantified