PA5-28809

antibody from Invitrogen Antibodies

Targeting: HIPK3 DYRK6, FIST3, PKY, YAK1

Western blot (Data presented on provider website)

Immunohistochemistry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-28809

Provider

Invitrogen Antibodies

Product name

HIPK3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Recommended positive controls: A431, H1299, HeLa, HepG2. Predicted reactivity: Mouse (100%), Rat (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.3 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Hsa_circ_0025202 suppresses cell tumorigenesis and tamoxifen resistance via miR-197-3p/HIPK3 axis in breast cancer.

Li H, Li Q, He S
World journal of surgical oncology 2021 Feb 3;19(1):39

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 6 HIPK3 is a target of miR-197-3p. a , b qRT-PCR and western blot analysis of HIPK3 expression in BC tissues and matched non-tumor tissues. c , d qRT-PCR and western blot analysis of HIPK3 expression in normal MCF-10A cells, BC cell lines (T47D and MCF7), and TAM-resistant BC cell lines (T47D/TAM and MCF7/TAM). e The correlation between HIPK3 level and miR-197-3p expression in BC tissues using the Pearson correlation analysis. f The potential binding sites of HIPK3 and miR-197-3p. g Luciferase activities detection in 293 T cells co-transfected with the reporter plasmids and indicated miRNAs using the dual-luciferase reporter assay. ** P < 0.01, *** P < 0.001, # P < 0.05, ## P < 0.01

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 7 MiR-197-3p promotes cell TAM resistance and carcinogenesis in BC via HIPK3. a , b HIPK3 level by qRT-PCR and western blot; c , d the IC 50 value of TAM by CCK-8 assay; e cell proliferation by colony formation assay; f cell cycle by flow cytometry assay; g cell invasion by transwell assay; h cell migration by wound healing assay; i cell apoptosis by flow cytometry; j protein levels of bax, ki-67, and bcl-2 by western blot, in T47D and MCF7 cells transfected with mimic NC, miR-197-3p mimic, miR-197-3p mimic + vector, or miR-197-3p mimic + oe-HIPK3. * P < 0.05, ** P < 0.01, *** P < 0.001