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Learn13683-1-AP
antibody from Proteintech Group
Targeting: CHMP4B
C20orf178, dJ553F4.4, Shax1, SNF7-2, VPS32B
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [23]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- 13683-1-AP - Provider product page
- Provider
- Proteintech Group
- Product name
- CHMP4B antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated CHMP4B antibody (Cat. #13683-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Mammalian hybrid pre-autophagosomal structure HyPAS generates autophagosomes.
Endofin is required for HD-PTP and ESCRT-0 interdependent endosomal sorting of ubiquitinated transmembrane cargoes.
His domain protein tyrosine phosphatase and Rabaptin-5 couple endo-lysosomal sorting of EGFR with endosomal maturation.
A conserved ubiquitin- and ESCRT-dependent pathway internalizes human lysosomal membrane proteins for degradation.
AKTIP interacts with ESCRT I and is needed for the recruitment of ESCRT III subunits to the midbody.
LRRK2 activation controls the repair of damaged endomembranes in macrophages.
Loss of MLKL (Mixed Lineage Kinase Domain-Like Protein) Decreases Necrotic Core but Increases Macrophage Lipid Accumulation in Atherosclerosis.
Restoration of lysosomal function after damage is accompanied by recycling of lysosomal membrane proteins.
Human Cytomegalovirus Utilizes Extracellular Vesicles To Enhance Virus Spread.
Synthetic Lethal Interaction between the ESCRT Paralog Enzymes VPS4A and VPS4B in Cancers Harboring Loss of Chromosome 18q or 16q.
Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.
Studying the Spatial Organization of ESCRTs in Cytokinetic Abscission Using the High-Resolution Imaging Techniques SIM and Cryo-SXT.
A charged multivesicular body protein (CHMP4B) is required for lens growth and differentiation.
A Septin Double Ring Controls the Spatiotemporal Organization of the ESCRT Machinery in Cytokinetic Abscission.
Vps4A mediates the localization and exosome release of β-catenin to inhibit epithelial-mesenchymal transition in hepatocellular carcinoma.
Nuclear envelope assembly defects link mitotic errors to chromothripsis.
iASPP-PP1 complex is required for cytokinetic abscission by controlling CEP55 dephosphorylation.
Nonenvelopment Role for the ESCRT-III Complex during Human Cytomegalovirus Infection.
Resolving ESCRT-III Spirals at the Intercellular Bridge of Dividing Cells Using 3D STORM.
Caspase-8 controls the secretion of inflammatory lysyl-tRNA synthetase in exosomes from cancer cells.
ESCRT proteins restrict constitutive NF-κB signaling by trafficking cytokine receptors.
ESCRT-0 marks an APPL1-independent transit route for EGFR between the cell surface and the EEA1-positive early endosome.
Recruitment of UBPY and ESCRT exchange drive HD-PTP-dependent sorting of EGFR to the MVB.
Kumar S, Javed R, Mudd M, Pallikkuth S, Lidke KA, Jain A, Tangavelou K, Gudmundsson SR, Ye C, Rusten TE, Anonsen JH, Lystad AH, Claude-Taupin A, Simonsen A, Salemi M, Phinney B, Li J, Guo LW, Bradfute SB, Timmins GS, Eskelinen EL, Deretic V
Cell 2021 Nov 24;184(24):5950-5969.e22
Cell 2021 Nov 24;184(24):5950-5969.e22
Endofin is required for HD-PTP and ESCRT-0 interdependent endosomal sorting of ubiquitinated transmembrane cargoes.
Kazan JM, Desrochers G, Martin CE, Jeong H, Kharitidi D, Apaja PM, Roldan A, St Denis N, Gingras AC, Lukacs GL, Pause A
iScience 2021 Nov 19;24(11):103274
iScience 2021 Nov 19;24(11):103274
His domain protein tyrosine phosphatase and Rabaptin-5 couple endo-lysosomal sorting of EGFR with endosomal maturation.
Parkinson G, Roboti P, Zhang L, Taylor S, Woodman P
Journal of cell science 2021 Nov 1;134(21)
Journal of cell science 2021 Nov 1;134(21)
A conserved ubiquitin- and ESCRT-dependent pathway internalizes human lysosomal membrane proteins for degradation.
Zhang W, Yang X, Chen L, Liu YY, Venkatarangan V, Reist L, Hanson P, Xu H, Wang Y, Li M
PLoS biology 2021 Jul;19(7):e3001361
PLoS biology 2021 Jul;19(7):e3001361
AKTIP interacts with ESCRT I and is needed for the recruitment of ESCRT III subunits to the midbody.
Merigliano C, Burla R, La Torre M, Del Giudice S, Teo H, Liew CW, Chojnowski A, Goh WI, Olmos Y, Maccaroni K, Giubettini M, Chiolo I, Carlton JG, Raimondo D, Vernì F, Stewart CL, Rhodes D, Wright GD, Burke BE, Saggio I
PLoS genetics 2021 Aug;17(8):e1009757
PLoS genetics 2021 Aug;17(8):e1009757
LRRK2 activation controls the repair of damaged endomembranes in macrophages.
Herbst S, Campbell P, Harvey J, Bernard EM, Papayannopoulos V, Wood NW, Morris HR, Gutierrez MG
The EMBO journal 2020 Sep 15;39(18):e104494
The EMBO journal 2020 Sep 15;39(18):e104494
Loss of MLKL (Mixed Lineage Kinase Domain-Like Protein) Decreases Necrotic Core but Increases Macrophage Lipid Accumulation in Atherosclerosis.
Rasheed A, Robichaud S, Nguyen MA, Geoffrion M, Wyatt H, Cottee ML, Dennison T, Pietrangelo A, Lee R, Lagace TA, Ouimet M, Rayner KJ
Arteriosclerosis, thrombosis, and vascular biology 2020 May;40(5):1155-1167
Arteriosclerosis, thrombosis, and vascular biology 2020 May;40(5):1155-1167
Restoration of lysosomal function after damage is accompanied by recycling of lysosomal membrane proteins.
Eriksson I, Wäster P, Öllinger K
Cell death & disease 2020 May 14;11(5):370
Cell death & disease 2020 May 14;11(5):370
Human Cytomegalovirus Utilizes Extracellular Vesicles To Enhance Virus Spread.
Streck NT, Zhao Y, Sundstrom JM, Buchkovich NJ
Journal of virology 2020 Jul 30;94(16)
Journal of virology 2020 Jul 30;94(16)
Synthetic Lethal Interaction between the ESCRT Paralog Enzymes VPS4A and VPS4B in Cancers Harboring Loss of Chromosome 18q or 16q.
Neggers JE, Paolella BR, Asfaw A, Rothberg MV, Skipper TA, Yang A, Kalekar RL, Krill-Burger JM, Dharia NV, Kugener G, Kalfon J, Yuan C, Dumont N, Gonzalez A, Abdusamad M, Li YY, Spurr LF, Wu WW, Durbin AD, Wolpin BM, Piccioni F, Root DE, Boehm JS, Cherniack AD, Tsherniak A, Hong AL, Hahn WC, Stegmaier K, Golub TR, Vazquez F, Aguirre AJ
Cell reports 2020 Dec 15;33(11):108493
Cell reports 2020 Dec 15;33(11):108493
Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.
Mercier V, Larios J, Molinard G, Goujon A, Matile S, Gruenberg J, Roux A
Nature cell biology 2020 Aug;22(8):947-959
Nature cell biology 2020 Aug;22(8):947-959
Studying the Spatial Organization of ESCRTs in Cytokinetic Abscission Using the High-Resolution Imaging Techniques SIM and Cryo-SXT.
Adar-Levor S, Goliand I, Elbaum M, Elia N
Methods in molecular biology (Clifton, N.J.) 2019;1998:129-148
Methods in molecular biology (Clifton, N.J.) 2019;1998:129-148
A charged multivesicular body protein (CHMP4B) is required for lens growth and differentiation.
Zhou Y, Bennett TM, Shiels A
Differentiation; research in biological diversity 2019 Sep-Oct;109:16-27
Differentiation; research in biological diversity 2019 Sep-Oct;109:16-27
A Septin Double Ring Controls the Spatiotemporal Organization of the ESCRT Machinery in Cytokinetic Abscission.
Karasmanis EP, Hwang D, Nakos K, Bowen JR, Angelis D, Spiliotis ET
Current biology : CB 2019 Jul 8;29(13):2174-2182.e7
Current biology : CB 2019 Jul 8;29(13):2174-2182.e7
Vps4A mediates the localization and exosome release of β-catenin to inhibit epithelial-mesenchymal transition in hepatocellular carcinoma.
Han Q, Lv L, Wei J, Lei X, Lin H, Li G, Cao J, Xie J, Yang W, Wu S, You J, Lu J, Liu P, Min J
Cancer letters 2019 Aug 10;457:47-59
Cancer letters 2019 Aug 10;457:47-59
Nuclear envelope assembly defects link mitotic errors to chromothripsis.
Liu S, Kwon M, Mannino M, Yang N, Renda F, Khodjakov A, Pellman D
Nature 2018 Sep;561(7724):551-555
Nature 2018 Sep;561(7724):551-555
iASPP-PP1 complex is required for cytokinetic abscission by controlling CEP55 dephosphorylation.
Gao K, Zhang Y, Shi Q, Zhang J, Zhang L, Sun H, Jiao D, Zhao X, Tao H, Wei Y, Wang Y, Saiyin H, Zhao SM, Li Y, Zhang P, Wang C
Cell death & disease 2018 May 1;9(5):528
Cell death & disease 2018 May 1;9(5):528
Nonenvelopment Role for the ESCRT-III Complex during Human Cytomegalovirus Infection.
Streck NT, Carmichael J, Buchkovich NJ
Journal of virology 2018 Jun 15;92(12)
Journal of virology 2018 Jun 15;92(12)
Resolving ESCRT-III Spirals at the Intercellular Bridge of Dividing Cells Using 3D STORM.
Goliand I, Adar-Levor S, Segal I, Nachmias D, Dadosh T, Kozlov MM, Elia N
Cell reports 2018 Aug 14;24(7):1756-1764
Cell reports 2018 Aug 14;24(7):1756-1764
Caspase-8 controls the secretion of inflammatory lysyl-tRNA synthetase in exosomes from cancer cells.
Kim SB, Kim HR, Park MC, Cho S, Goughnour PC, Han D, Yoon I, Kim Y, Kang T, Song E, Kim P, Choi H, Mun JY, Song C, Lee S, Jung HS, Kim S
The Journal of cell biology 2017 Jul 3;216(7):2201-2216
The Journal of cell biology 2017 Jul 3;216(7):2201-2216
ESCRT proteins restrict constitutive NF-κB signaling by trafficking cytokine receptors.
Mamińska A, Bartosik A, Banach-Orłowska M, Pilecka I, Jastrzębski K, Zdżalik-Bielecka D, Castanon I, Poulain M, Neyen C, Wolińska-Nizioł L, Toruń A, Szymańska E, Kowalczyk A, Piwocka K, Simonsen A, Stenmark H, Fürthauer M, González-Gaitán M, Miaczynska M
Science signaling 2016 Jan 19;9(411):ra8
Science signaling 2016 Jan 19;9(411):ra8
ESCRT-0 marks an APPL1-independent transit route for EGFR between the cell surface and the EEA1-positive early endosome.
Flores-Rodriguez N, Kenwright DA, Chung PH, Harrison AW, Stefani F, Waigh TA, Allan VJ, Woodman PG
Journal of cell science 2015 Feb 15;128(4):755-67
Journal of cell science 2015 Feb 15;128(4):755-67
Recruitment of UBPY and ESCRT exchange drive HD-PTP-dependent sorting of EGFR to the MVB.
Ali N, Zhang L, Taylor S, Mironov A, Urbé S, Woodman P
Current biology : CB 2013 Mar 18;23(6):453-61
Current biology : CB 2013 Mar 18;23(6):453-61
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- mouse heart tissue were subjected to SDS PAGE followed by western blot with 13683-1-AP(CHMP4B antibody) at dilution of 1:200
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human oesophagus cancer using 13683-1-AP(CHMP4B antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The CHMP4B antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human CHMP4B. This antibody recognizes human, mouse, rat antigen. The CHMP4B antibody has been validated for the following applications: ELISA, WB, IHC analysis.
