Explore
Validate
Learn
Western blot
ELISA
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunoprecipitation [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-148244 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ARF3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 100 µg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of ARF3 in Hela cells. Samples were incubated with ARF3 polyclonal antibody (Product # PA5-148244) using a dilution of 1:200 at 4°C overnight followed by Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Cells were fixed with 4% PFA, permeabilized with 0.3% Triton X-100 in PBS, and blocked with 10% serum. Counterstained with DAPI (blue). Positive staining was localized to Cytoplasm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of ARF3 in various lysates. Samples were incubated with ARF3 polyclonal antibody (Product # PA5-148244) using a dilution of 1:100 followed by Goat Anti-Rabbit IgG (H+L)/HRP secondary antibody at a dilution of 1:10,000. Lane A: 0.5 mg HeLa Whole Cell Lysate; Lane B: 0.5 mg HepG2 Whole Cell Lysate; Lane C: 0.5 mg Raji Whole Cell Lysate; Lane D: 0.5 mg MCF7 Whole Cell Lysate. 4 µL anti- ARF3 rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein A/G. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 19 kDa; Observed band size: 19 kDa.
