MA5-50467

antibody from Invitrogen Antibodies

Targeting: CACNG2 MGC138502, MGC138504, stargazin

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-50467

Provider

Invitrogen Antibodies

Product name

Stargazin Recombinant Rabbit Monoclonal Antibody (HL2268)

Antibody type

Monoclonal

Antigen

Recombinant protein fragment

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

HL2268

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of Stargazin using Stargazin Recombinant Monoclonal Antibody (Product # MA5-50467) in Mouse brain and Mouse spleen samples using a dilution of 1:1,000. Extracts were separated by 10% SDS-PAGE. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of Stargazin using Stargazin Recombinant Monoclonal Antibody (Product # MA5-50467) in Rat brain tissue extract (50 μg) at a dilution of 1:1,000. Extracts were separated by 10% SDS PAGE. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of Stargazin using Stargazin Recombinant Monoclonal Antibody (Product # MA5-50467) in SH-SY5Y cells at a dilution of 1:500. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Stargazin stained by Stargazin antibody (MA5-50467) diluted at 1:500. Blue: Fluoroshield with DAPI.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of Stargazin using Stargazin Recombinant Monoclonal Antibody (Product # MA5-50467) in DIV9 rat E18 primary hippocampal neuron cells at a dilution of 1:250. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Stargazin stained by Stargazin antibody (MA5-50467) diluted at 1:250. Red: Tau, a axon marker, stained by Tau antibody diluted at 1:500. Blue: Fluoroshield with DAPI.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Stargazin using Stargazin Recombinant Monoclonal Antibody (Product # MA5-50467) in Paraffin embedded mouse brain and cerebellum tissue at a dilution of 1:100. For Antigen retrieval citrate buffer, pH 6.0, as used for 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Stargazin using Stargazin Recombinant Monoclonal Antibody (Product # MA5-50467) in Paraffin embedded rat brain tissue at a dilution of 1:100. For Antigen retrieval citrate buffer, pH 6.0, as used for 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Stargazin using Stargazin Recombinant Monoclonal Antibody (Product # MA5-50467) in Paraffin embedded rat brain tissue at a dilution of 1:100. For Antigen retrieval citrate buffer, pH 6.0, as used for 15 min.