Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [2]
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Validation data
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- Product number
- ALX-210-940-C100 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ALX-210-940-C100, RRID:AB_2052562
- Product name
- SLP-2 polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Protein A-affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100 μg
- Storage
- -20°C
- Handling
- Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20¡C.
Submitted references SLP-2 is required for stress-induced mitochondrial hyperfusion.
Tondera D, Grandemange S, Jourdain A, Karbowski M, Mattenberger Y, Herzig S, Da Cruz S, Clerc P, Raschke I, Merkwirth C, Ehses S, Krause F, Chan DC, Alexander C, Bauer C, Youle R, Langer T, Martinou JC
The EMBO journal 2009 Jun 3;28(11):1589-600
The EMBO journal 2009 Jun 3;28(11):1589-600
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Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Endogenous SLP-2 is localized to the mitochondria. Method: Endogenous SLP-2 (green) (up) and endogenous mitochondrial HSP70 (red) (middle) were detected in methanol fixed HeLa cells using PAb to SLP-2 (Prod. No. ALX-210-940) (1:100) and a PAb to mitochondrial HSP70, respectively. Picture courtesy of Dr. Sandrine Da Cruz, University of Geneva.
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Detection of endogenous SLP-2 using PAb to SLP-2 (Prod. No. ALX-210-940). Method: Total protein extracts from HeLa cell line were run on SDS-PAGE and SLP-2 was detected by PAb to SLP-2 at 1:1'000 dilution. Anti-rabbit IgG coupled horseradish peroxidase was used at 1:5'000 dilution for ECL detection. Picture courtesy of Prof. Jean-Claude Martinou.