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ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- RQ6003 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- PTF1A Antibody
- Antibody type
- Polyclonal
- Description
- This highly specific PTF1A antibody is suitable for use in Western blot/Immunofluorescence/Flow cytometry/Direct ELISA applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the PTF1A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) rat pancreas, 2) mouse pancreas, 3) human PANC-1, 4) human PANC-1, 5) human U-2 OS and 6) mouse SP2/0 lysate with PTF1A antibody. Predicted molecular weight: ~35 kDa, routinely observed at ~48 kDa. (Ref 1)
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human A431 cells with PTF1A antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human A431 cells with PTF1A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PTF1A antibody.
