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Western blot
Immunoprecipitation
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [2]
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- Product number
- PA5-28469 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- eIF2 beta Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLa, mouse liver, PC-12, Rat2. Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (98%), Xenopus laevis (98%), Rabbit (100%), Chicken (99%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using eIF2 beta Polyclonal Antibody (Product # PA5-28469). Sample (30 µg of whole cell lysate). Lane A: 293T. Lane B: A431. Lane C: H1299. Lane D: Hela . 10% SDS PAGE. eIF2 beta Polyclonal Antibody (Product # PA5-28469) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using eIF2 beta Polyclonal Antibody (Product # PA5-28469). Sample (50 µg of whole cell lysate). Lane A: mouse liver. 10% SDS PAGE. eIF2 beta Polyclonal Antibody (Product # PA5-28469) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- eIF2 beta Polyclonal Antibody detects EIF2S2 protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract. B. 30 µg Rat2 whole cell lysate/extract.10 % SDS-PAGE. EIF2 beta Polyclonal Antibody (Product # PA5-28469) dilution: 1:2,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- eIF2 beta Polyclonal Antibody detects EIF2S2 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EIF2S2 protein stained by eIF2 beta Polyclonal Antibody (Product # PA5-28469) diluted at 1:500. Blue: Hoechst 33343 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- eIF2 beta Polyclonal Antibody detects EIF2S2 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EIF2S2 protein stained by eIF2 beta Polyclonal Antibody (Product # PA5-28469) diluted at 1:500. Blue: Hoechst 33343 staining.
