NB100-93284

antibody from Novus Biologicals

Targeting: EIF2S2 EIF2, EIF2beta, PPP1R67

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB100-93284

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-93284, RRID:AB_1236901

Product name

Rabbit Polyclonal EIF2 beta Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Concentration

0.2 mg/ml

Storage

Store at 4C. Do not freeze.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: EIF2 beta Antibody [NB100-93284] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. NB100-93284 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. eIF2beta/EIF2S2 was immunoprecipitated less efficiently by NB100-93283, which recognizes an upstream epitope

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: EIF2 beta Antibody [NB100-93284] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-eIF2beta/EIF2S2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB