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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
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- Product number
- TA347233 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal Mll4 Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal Mll4 Antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- KMT2D
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µl
- Concentration
- not determined
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- WB was performed on whole cell lysates from mouse embryonic stem cells (E14Tg2a) with the mouse Mll4 ab, diluted 1:500 in PBS-Tween with 5% skimmed milk. The location of the protein of interest (~ 600 kDa) is indicated on the right; the location of the 500 kDa MW marker is shown on the left. Cells homozygous for the targeted mll4 allele (-/-) show a complete loss of Mll4 protein, whereas the protein was detected in flp-recombined ES cells (F/F) and heterozygously targeted ES cells (+/-).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody against mouse Mll4. The wells were coated with the peptides used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:15,000.
- Validation comment
- ELISA
