13365-1-AP

antibody from Proteintech Group

Targeting: NAT10 FLJ10774, FLJ12179, hALP, KIAA1709, Kre33, NET43

Provider product page for 13365-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Antibody data

Antibody Data
Antigen structure
References [52]
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Validations
Western blot [1]
Immunocytochemistry [1]

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Product number: 13365-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#13365-1-AP, RRID:AB_2148944
Product name: NAT10 antibody
Antibody type: Polyclonal
Description: KD/KO validated NAT10 antibody (Cat. #13365-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IP, WB, ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

NAT10 protein structure - 13365-1-AP shown in red.

Submitted references A personalized biomimetic dual-drug delivery system via controlled release of PTH(1-34) and simvastatin for in situ osteoporotic bone regeneration.

Xu T, Gao S, Yang N, Zhao Q, Zhang Y, Li T, Liu Z, Han B
Frontiers in bioengineering and biotechnology 2024;12:1355019

NAT10/CEBPB/vimentin signalling axis promotes adenoid cystic carcinoma malignant phenotypes in vitro.

Fu M, Gao Q, Xiao M, Sun XY, Li SL, Ge XY
Oral diseases 2024 Oct;30(7):4341-4355

Acetyl-CoA-dependent ac(4)C acetylation promotes the osteogenic differentiation of LPS-stimulated BMSCs.

Bai Y, Zhang W, Hao L, Zhao Y, Tsai IC, Qi Y, Xu Q
International immunopharmacology 2024 May 30;133:112124

N-Acetyltransferase 10 represses Uqcr11 and Uqcrb independently of ac4C modification to promote heart regeneration.

Ma W, Tian Y, Shi L, Liang J, Ouyang Q, Li J, Chen H, Sun H, Ji H, Liu X, Huang W, Gao X, Jin X, Wang X, Liu Y, Yu Y, Guo X, Tian Y, Yang F, Li F, Wang N, Cai B
Nature communications 2024 Mar 8;15(1):2137

Artesunate-loaded thermosensitive chitosan hydrogel promotes osteogenesis of maxillary tooth extraction through regulating T lymphocytes in type 2 diabetic rats.

Luo J, Liang C, Chen K, Zeng K, Bai R, Tang C, Li J, Nong X
BMC oral health 2024 Mar 20;24(1):356

Arf1 GTPase Regulates Golgi-Dependent G2/M Transition and Spindle Organization in Oocyte Meiosis.

Zhang KH, Zou YJ, Shan MM, Pan ZN, Ju JQ, Liu JC, Ji YM, Sun SC
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2024 Jan;11(4):e2303009

CLIC3 interacts with NAT10 to inhibit N4-acetylcytidine modification of p21 mRNA and promote bladder cancer progression.

Shuai Y, Zhang H, Liu C, Wang J, Jiang Y, Sun J, Gao X, Bo X, Xiao X, Liao X, Huang C, Chen H, Jiang G
Cell death & disease 2024 Jan 5;15(1):9

N-acetyltransferase 10 regulates alphavirus replication via N4-acetylcytidine (ac4C) modiﬁcation of the lymphocyte antigen six family member E (LY6E) mRNA.

Dang Y, Li J, Li Y, Wang Y, Zhao Y, Zhao N, Li W, Zhang H, Ye C, Ma H, Zhang L, Liu H, Dong Y, Yao M, Lei Y, Xu Z, Zhang F, Ye W
Journal of virology 2024 Jan 23;98(1):e0135023

RNPS1 stabilizes NAT10 protein to facilitate translation in cancer via tRNA ac(4)C modification.

Wang X, Ling R, Peng Y, Qiu W, Chen D
International journal of oral science 2024 Jan 22;16(1):6

Targeting the NAT10/NPM1 axis abrogates PD-L1 expression and improves the response to immune checkpoint blockade therapy.

Qin G, Bai F, Hu H, Zhang J, Zhan W, Wu Z, Li J, Fu Y, Deng Y
Molecular medicine (Cambridge, Mass.) 2024 Jan 20;30(1):13

Deficiency of Acetyltransferase nat10 in Zebrafish Causes Developmental Defects in the Visual Function.

Yang HZ, Zhuo D, Huang Z, Luo G, Liang S, Fan Y, Zhao Y, Lv X, Qiu C, Zhang L, Liu Y, Sun T, Chen X, Li SS, Jin X
Investigative ophthalmology & visual science 2024 Feb 1;65(2):31

Peptidylprolyl isomerase D circular RNA sensitizes breast cancer to trastuzumab through remodeling HER2 N4-acetylcytidine modification.

Wang S, Li Q, Wang Y, Li X, Feng X, Wei Y, Wang J, Zhou X
Journal of applied genetics 2024 Dec;65(4):797-807

N4-acetylcytidine (ac4C) promotes mRNA localization to stress granules.

Kudrin P, Singh A, Meierhofer D, Kuśnierczyk A, Ørom UAV
EMBO reports 2024 Apr;25(4):1814-1834

Activated SIRT1 contributes to DPT-induced glioma cell parthanatos by upregulation of NOX2 and NAT10.

Liang SP, Wang XZ, Piao MH, Chen X, Wang ZC, Li C, Wang YB, Lu S, He C, Wang YL, Chi GF, Ge PF
Acta pharmacologica Sinica 2023 Oct;44(10):2125-2138

NAT10 regulates the repair of UVB-induced DNA damage and tumorigenicity.

Yang Z, Wilkinson E, Cui YH, Li H, He YY
Toxicology and applied pharmacology 2023 Oct 15;477:116688

Lysine 2-hydroxyisobutyrylation of NAT10 promotes cancer metastasis in an ac4C-dependent manner.

Liao L, He Y, Li SJ, Yu XM, Liu ZC, Liang YY, Yang H, Yang J, Zhang GG, Deng CM, Wei X, Zhu YD, Xu TY, Zheng CC, Cheng C, Li A, Li ZG, Liu JB, Li B
Cell research 2023 May;33(5):355-371

Maternal NAT10 orchestrates oocyte meiotic cell-cycle progression and maturation in mice.

Jiang X, Cheng Y, Zhu Y, Xu C, Li Q, Xing X, Li W, Zou J, Meng L, Azhar M, Cao Y, Tong X, Qin W, Zhu X, Bao J
Nature communications 2023 Jun 22;14(1):3729

Exosomes promote hFOB1.19 proliferation and differentiation via LINC00520.

Wu J, Zhang L, Liu H, Zhang J, Tang P
Journal of orthopaedic surgery and research 2023 Jul 29;18(1):546

NAT10-mediated ac4C tRNA modification promotes EGFR mRNA translation and gefitinib resistance in cancer.

Wei W, Zhang S, Han H, Wang X, Zheng S, Wang Z, Yang C, Wang L, Ma J, Guo S, Wang J, Liu L, Choe J, Lin S
Cell reports 2023 Jul 25;42(7):112810

NAT10 promotes the tumorigenesis and progression of laryngeal squamous cell carcinoma through ac4C modification of FOXM1 mRNA.

Li Z, Li D, Yang T, Yao C
Cancer biology & therapy 2023 Dec 31;24(1):2274143

NAT10 Maintains OGA mRNA Stability Through ac4C Modification in Regulating Oocyte Maturation.

Lin J, Xiang Y, Huang J, Zeng H, Zeng Y, Liu J, Wu T, Liang Q, Liang X, Li J, Zhou C
Frontiers in endocrinology 2022;13:907286

NAT10 acetylates BCL-XL mRNA to promote the proliferation of multiple myeloma cells through PI3K-AKT pathway.

Zhang Y, Deng Z, Sun S, Xie S, Jiang M, Chen B, Gu C, Yang Y
Frontiers in oncology 2022;12:967811

N4-acetylcytidine regulates the replication and pathogenicity of enterovirus 71.

Hao H, Liu W, Miao Y, Ma L, Yu B, Liu L, Yang C, Zhang K, Chen Z, Yang J, Zheng Z, Zhang B, Deng F, Gong P, Yuan J, Hu Z, Guan W
Nucleic acids research 2022 Sep 9;50(16):9339-9354

NAT10-mediated N4-acetylcytidine modification is required for meiosis entry and progression in male germ cells.

Chen L, Wang WJ, Liu Q, Wu YK, Wu YW, Jiang Y, Liao XQ, Huang F, Li Y, Shen L, Yu C, Zhang SY, Yan LY, Qiao J, Sha QQ, Fan HY
Nucleic acids research 2022 Oct 28;50(19):10896-10913

Mechanical Force Modulates Alveolar Bone Marrow Mesenchymal Cells Characteristics for Bone Remodeling during Orthodontic Tooth Movement through Lactate Production.

Zhai M, Cui S, Li L, Cheng C, Zhang Z, Liu J, Wei F
Cells 2022 Nov 22;11(23)

Ginsenoside Rc Promotes Bone Formation in Ovariectomy-Induced Osteoporosis In Vivo and Osteogenic Differentiation In Vitro.

Yang N, Zhang X, Li L, Xu T, Li M, Zhao Q, Yu J, Wang J, Liu Z
International journal of molecular sciences 2022 May 31;23(11)

Facile Fabrication of 3D-Printed Porous Ti6Al4V Scaffolds with a Sr-CaP Coating for Bone Regeneration.

Su S, Chen W, Zheng M, Lu G, Tang W, Huang H, Qu D
ACS omega 2022 Mar 15;7(10):8391-8402

Kinesin motor KIFC1 is required for tubulin acetylation and actin-dependent spindle migration in mouse oocyte meiosis.

Shan MM, Zou YJ, Pan ZN, Zhang HL, Xu Y, Ju JQ, Sun SC
Development (Cambridge, England) 2022 Mar 1;149(5)

Elevation of N-acetyltransferase 10 in hippocampal neurons mediates depression- and anxiety-like behaviors.

Guo XF, Wang XH, Fu YL, Meng Q, Huang BY, Yang R, Guo Y, Du YR, Wang X, Gao Y, Song L, Gong M, Wang S, Li YD, Shi HS, Shi Y
Brain research bulletin 2022 Jul;185:91-98

Kinesin KIF15 regulates tubulin acetylation and spindle assembly checkpoint in mouse oocyte meiosis.

Zou YJ, Shan MM, Wan X, Liu JC, Zhang KH, Ju JQ, Xing CH, Sun SC
Cellular and molecular life sciences : CMLS 2022 Jul 14;79(8):422

Hydroxyapatite-Tethered Peptide Hydrogel Promotes Osteogenesis.

Yu H, Song J, Zhang X, Jiang K, Fan H, Li Y, Zhao Y, Liu S, Hao D, Li G
Gels (Basel, Switzerland) 2022 Dec 8;8(12)

NAT10 promotes cell proliferation by acetylating CEP170 mRNA to enhance translation efficiency in multiple myeloma.

Wei R, Cui X, Min J, Lin Z, Zhou Y, Guo M, An X, Liu H, Janz S, Gu C, Wang H, Yang Y
Acta pharmaceutica Sinica. B 2022 Aug;12(8):3313-3325

Direct epitranscriptomic regulation of mammalian translation initiation through N4-acetylcytidine.

Arango D, Sturgill D, Yang R, Kanai T, Bauer P, Roy J, Wang Z, Hosogane M, Schiffers S, Oberdoerffer S
Molecular cell 2022 Aug 4;82(15):2797-2814.e11

NAT10-Mediated N4-Acetylcytidine of RNA Contributes to Post-transcriptional Regulation of Mouse Oocyte Maturation in vitro.

Xiang Y, Zhou C, Zeng Y, Guo Q, Huang J, Wu T, Liu J, Liang Q, Zeng H, Liang X
Frontiers in cell and developmental biology 2021;9:704341

Dura cells in the etiopathogenesis of Crouzon syndrome: the effects of FGFR2 mutations in the dura cells on the proliferation of osteoblasts through the hippo/YAP mediated transcriptional regulation pathway.

Dong XH, Zhang MZ, Lai CZ, Li CC, Du L, Song GD, Zong XL, Jin XL
American journal of translational research 2021;13(10):11255-11270

Prognostic and Immunological Role of mRNA ac4C Regulator NAT10 in Pan-Cancer: New Territory for Cancer Research?

Yang C, Wu T, Zhang J, Liu J, Zhao K, Sun W, Zhou X, Kong X, Shi J
Frontiers in oncology 2021;11:630417

Multiple Omics Integration Reveals Key Circular RNAs in Hepatocellular Carcinoma.

Huang ZL, Huang XY, Huang J, Huang XY, Xu YH, Zhou J, Tang ZY
Frontiers in oncology 2021;11:621353

Single-Cell RNA-Sequencing Reveals the Breadth of Osteoblast Heterogeneity.

Yoshioka H, Okita S, Nakano M, Minamizaki T, Nubukiyo A, Sotomaru Y, Bonnelye E, Kozai K, Tanimoto K, Aubin JE, Yoshiko Y
JBMR plus 2021 Jun;5(6):e10496

ac4C acetylation of RUNX2 catalyzed by NAT10 spurs osteogenesis of BMSCs and prevents ovariectomy-induced bone loss.

Yang W, Li HY, Wu YF, Mi RJ, Liu WZ, Shen X, Lu YX, Jiang YH, Ma MJ, Shen HY
Molecular therapy. Nucleic acids 2021 Dec 3;26:135-147

Knee loading repairs osteoporotic osteoarthritis by relieving abnormal remodeling of subchondral bone via Wnt/β-catenin signaling.

Zheng W, Ding B, Li X, Liu D, Yokota H, Zhang P
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 Feb;34(2):3399-3412

Acetylation of Cytidine Residues Boosts HIV-1 Gene Expression by Increasing Viral RNA Stability.

Tsai K, Jaguva Vasudevan AA, Martinez Campos C, Emery A, Swanstrom R, Cullen BR
Cell host & microbe 2020 Aug 12;28(2):306-312.e6

CCDC84 Acetylation Oscillation Regulates Centrosome Duplication by Modulating HsSAS-6 Degradation.

Wang T, Zou Y, Huang N, Teng J, Chen J
Cell reports 2019 Nov 12;29(7):2078-2091.e5

MicroRNA‑223 promotes osteoblast differentiation of MC3T3‑E1 cells by targeting histone deacetylase 2.

Chen J, He G, Wang Y, Cai D
International journal of molecular medicine 2019 Mar;43(3):1513-1521

Small molecule inhibitor of TGF-β signaling enables robust osteogenesis of autologous GMSCs to successfully repair minipig severe maxillofacial bone defects.

Shi A, Heinayati A, Bao D, Liu H, Ding X, Tong X, Wang L, Wang B, Qin H
Stem cell research & therapy 2019 Jun 13;10(1):172

RAB35 depletion affects spindle formation and actin-based spindle migration in mouse oocyte meiosis.

Zhang Y, Wan X, Wang HH, Pan MH, Pan ZN, Sun SC
Molecular human reproduction 2019 Jul 1;25(7):359-372

Rab23/Kif17 regulate meiotic progression in oocytes by modulating tubulin acetylation and actin dynamics.

Wang HH, Zhang Y, Tang F, Pan MH, Wan X, Li XH, Sun SC
Development (Cambridge, England) 2019 Feb 4;146(3)

Kif18a regulates Sirt2-mediated tubulin acetylation for spindle organization during mouse oocyte meiosis.

Tang F, Pan MH, Wan X, Lu Y, Zhang Y, Sun SC
Cell division 2018;13:9

Acetylation of Cytidine in mRNA Promotes Translation Efficiency.

Arango D, Sturgill D, Alhusaini N, Dillman AA, Sweet TJ, Hanson G, Hosogane M, Sinclair WR, Nanan KK, Mandler MD, Fox SD, Zengeya TT, Andresson T, Meier JL, Coller J, Oberdoerffer S
Cell 2018 Dec 13;175(7):1872-1886.e24

Targeting of NAT10 enhances healthspan in a mouse model of human accelerated aging syndrome.

Balmus G, Larrieu D, Barros AC, Collins C, Abrudan M, Demir M, Geisler NJ, Lelliott CJ, White JK, Karp NA, Atkinson J, Kirton A, Jacobsen M, Clift D, Rodriguez R, Sanger Mouse Genetics Project, Adams DJ, Jackson SP
Nature communications 2018 Apr 27;9(1):1700

Human GW182 Paralogs Are the Central Organizers for RNA-Mediated Control of Transcription.

Hicks JA, Li L, Matsui M, Chu Y, Volkov O, Johnson KC, Corey DR
Cell reports 2017 Aug 15;20(7):1543-1552

RCCD1 depletion attenuates TGF-β-induced EMT and cell migration by stabilizing cytoskeletal microtubules in NSCLC cells.

Wu J, He Z, Yang XM, Li KL, Wang DL, Sun FL
Cancer letters 2017 Aug 1;400:18-29

Chemical inhibition of NAT10 corrects defects of laminopathic cells.

Larrieu D, Britton S, Demir M, Rodriguez R, Jackson SP
Science (New York, N.Y.) 2014 May 2;344(6183):527-32

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: mouse testis tissue were subjected to SDS PAGE followed by western blot with 13365-1-AP(NAT10 antibody) at dilution of 1:500
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of Hela cells, using NAT10 antibody 13365-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Sample type: cell line