LS-C675075

antibody from LSBio

Targeting: NAT10 FLJ10774, FLJ12179, hALP, KIAA1709, Kre33, NET43

Western blot (Enhanced validation)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-C675075

Provider

LSBio

Product name

NAT10 Antibody LS-C675075

Antibody type

Polyclonal

Description

Protein G purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western Blot Positive WB detected in: Hela whole cell lysate All lanes:NAT10 antibody at 7µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 116, 108 kDa Observed band size: 116 kDa

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western Blot Positive WB detected in: Hela whole cell lysate All lanes:NAT10 antibody at 7µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 116, 108 kDa Observed band size: 116 kDa

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitating NAT10 in Hela whole cell lysate Lane 1: Rabbit control IgG instead of NAT10 Antibody in Hela whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: NAT10 Antibody (6µg) + Hela whole cell lysate (1mg) Lane 3: Hela whole cell lysate (20µg)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating NAT10 in Hela whole cell lysate Lane 1: Rabbit control IgG instead of NAT10 Antibody in Hela whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: NAT10 Antibody (6µg) + Hela whole cell lysate (1mg) Lane 3: Hela whole cell lysate (20µg)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating NAT10 in Hela whole cell lysate Lane 1: Rabbit control IgG instead of NAT10 Antibody in Hela whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: NAT10 Antibody (6µg) + Hela whole cell lysate (1mg) Lane 3: Hela whole cell lysate (20µg)