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Western blot
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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [3]
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- Product number
- LS-C676773 - Provider product page
- Provider
- LSBio
- Product name
- HIST1H2BN Antibody LS-C676773
- Antibody type
- Polyclonal
- Description
- Immunoaffinity purified
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western Blot Positive WB detected in: K562 whole cell lysate, 293 whole cell lysate(all treated with 30mM sodium butyrate for 4h) All Lanes: HIST1H2BC antibody at 1.85µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 14 KDa Observed band size: 14 KDa
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western Blot Detected samples: Hela whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, 293 whole cell lysate; Untreated (-) or treated (+) with 30mM sodium butyrate for 4h All lanes: HIST1H2BC antibody at 1:500 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 14 kDa Observed band size: 14 kDa
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunocytochemistry analysis diluted at 1:15 and staining in Hela cells(treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis diluted at 1:15 and staining in Hela cells(treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis diluted at 1:15 and staining in Hela cells(treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
