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LearnA304-373A
antibody from Invitrogen Antibodies
Targeting: PCLAF
KIAA0101, NS5ATP9, OEATC-1, p15(PAF), PAF15
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- A304-373A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PAF Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- The recommended shelf life for this product is 1 year from date of receipt.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human PAF by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- PAF (Cat. No. A304-373A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human PAF by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using RIPA buffer from HeLa cells. Antibodies: Affinity purified rabbit anti-PAF antibody A304-373A (lot A304-373A-1) used for IP at 6 µg per reaction. PAF was also immunoprecipitated by rabbit anti-PAF antibody BL15806. For blotting immunoprecipitated PAF, A304-373A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
