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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
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- Product number
- AF1928 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Spinesin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse Spinesin in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant human Spinesin is observed.
- Reactivity
- Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Spinesin in Mouse Brain. Spinesin was detected in perfusion fixed frozen sections of mouse brain (cerebellum) using Goat Anti-Mouse Spinesin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1928) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of neurons in the granular cell layer. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
