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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
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- Product number
- PA5-118696 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-JDP2 (Thr148) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Antibody detects endogenous levels of JDP2 only when phosphorylated at Thr148.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunocytochemical analysis of Phospho-JDP2 (Thr148) in Hela cells (treated for 4 hours with LPS) using a Phospho-JDP2 (Thr148) polyclonal antibody (Product # PA5-118696). The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with the primary antibody and mouse anti-beta tubulin antibody at a dilution of 1:200 for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).
