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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-22175 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EEF2K Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431, H1299, HeLa, HepG2, Raji, mouse liver, PC-12.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of EEF2K was performed by separating 30 µg of Whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a EEF2K Polyclonal Antibody (Product # PA5-22175) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using EEF2K Polyclonal Antibody (Product # PA5-22175). Sample (30 µg of whole cell lysate). Lane A: A431. Lane B: H1299. Lane C: Hela . Lane D: Hep G2 . 7.5% SDS PAGE. EEF2K Polyclonal Antibody (Product # PA5-22175) diluted at 1:5,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using EEF2K Polyclonal Antibody (Product # PA5-22175). Sample (50 µg of whole cell lysate). Lane A: mouse liver . 7.5% SDS PAGE. EEF2K Polyclonal Antibody (Product # PA5-22175) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of EEF2K in paraformaldehyde-fixed A431 cells using an EEF2K polyclonal antibody (Product # PA5-22175) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using eEF-2K (Product # PA5-22175) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
