NB100-87019

antibody from Novus Biologicals

Targeting: EEF2K CaMKIII, eEF-2K

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB100-87019

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-87019, RRID:AB_1199446

Product name

Rabbit Polyclonal EEF2K Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Concentration

1.0 mg/ml

Storage

Store at 4C. Do not freeze.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: EEF2K Antibody [NB100-87019] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-87019 used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. eEF2 Kinase was successfully immunoprecipitated by NB100-87018, which recognizes an upstream epitope.

Immunocytochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence: EEF2K Antibody [NB100-87019] - FFPE section of human colon carcinoma. Affinity purified rabbit anti-eEF2 Kinase used at a dilution of 1:100.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: EEF2K Antibody [NB100-87019] - Section of human stomach carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-eEF2 Kinase used at a dilution of 1:1,000 (1ug/ml). Detection: DAB