H00010538-M01

antibody from Abnova Corporation

Targeting: BATF B-ATF, BATF1, SFA-2

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

H00010538-M01

Provider

Abnova Corporation

Proper citation

Product name

BATF monoclonal antibody (M01), clone 8A12

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant BATF.

Antigen sequence

EKNRIAAQKSRQRQTQKADTLHLESEDLEKQNAAL
RKEIKQLTEELKYFTSVLNSHEPLCSVLAASTPSP
PEVVYSAHAFHQPHVSSPRFQP


Isotype

IgG

Antibody clone number

8A12

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Basic leucine zipper transcription factor, ATF-like (BATF) regulates epigenetically and energetically effector CD8 T-cell differentiation via Sirt1 expression.

Kuroda S, Yamazaki M, Abe M, Sakimura K, Takayanagi H, Iwai Y
Proceedings of the National Academy of Sciences of the United States of America 2011 Sep 6;108(36):14885-9

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

BATF monoclonal antibody (M01), clone 8A12 Western Blot analysis of BATF expression in Hela S3 NE ( Cat # L013V3 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

BATF monoclonal antibody (M01), clone 8A12. Western Blot analysis of BATF expression in Raw 264.7 ( Cat # L024V1 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of BATF expression in transfected 293T cell line by BATF monoclonal antibody (M01), clone 8A12.Lane 1: BATF transfected lysate(14.1 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged BATF is approximately 1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunoprecipitation

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoprecipitation of BATF transfected lysate using anti-BATF monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with BATF MaxPab rabbit polyclonal antibody.

Validation comment

Immunoprecipitation

Protocol

Protocol