Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 50-9860-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BATF Monoclonal Antibody (MBM7C7), eFluor™ 660, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The MBM7C7 monoclonal antibody reacts with human and mouse BATF (B cell activating transcription factor). BATF is a 14 kDa member of the AP-1 family of transcription factors and is involved in the repression of other AP-1 family members. BATF is upregulated in activated T cells when compared to naive T and B cells and has been shown to be critical for Th17, but not Th1 or Th2 differentiation. In Th17, BATF interacts with the promoters of IL17, IL17F, IL21 and IL22. BATF has also been shown to be involved in CD8 T cell exhaustion in HIV infection. Applications Reported: This MBM7C7 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This MBM7C7 antibody has been tested by intracellular staining and flow cytometric analysis using the Foxp3/Transcription Factor Buffer Set (Product # 00-5523-00) and protocol. Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- MBM7C7
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Regulation of TH17 cell differentiation by innate immune signals.
The AP-1 transcription factor Batf controls T(H)17 differentiation.
BATF transgenic mice reveal a role for activator protein-1 in NKT cell development.
Characterization of murine BATF: a negative regulator of activator protein-1 activity in the thymus.
Huang G, Wang Y, Chi H
Cellular & molecular immunology 2012 Jul;9(4):287-95
Cellular & molecular immunology 2012 Jul;9(4):287-95
The AP-1 transcription factor Batf controls T(H)17 differentiation.
Schraml BU, Hildner K, Ise W, Lee WL, Smith WA, Solomon B, Sahota G, Sim J, Mukasa R, Cemerski S, Hatton RD, Stormo GD, Weaver CT, Russell JH, Murphy TL, Murphy KM
Nature 2009 Jul 16;460(7253):405-9
Nature 2009 Jul 16;460(7253):405-9
BATF transgenic mice reveal a role for activator protein-1 in NKT cell development.
Williams KL, Zullo AJ, Kaplan MH, Brutkiewicz RR, Deppmann CD, Vinson C, Taparowsky EJ
Journal of immunology (Baltimore, Md. : 1950) 2003 Mar 1;170(5):2417-26
Journal of immunology (Baltimore, Md. : 1950) 2003 Mar 1;170(5):2417-26
Characterization of murine BATF: a negative regulator of activator protein-1 activity in the thymus.
Williams KL, Nanda I, Lyons GE, Kuo CT, Schmid M, Leiden JM, Kaplan MH, Taparowsky EJ
European journal of immunology 2001 May;31(5):1620-7
European journal of immunology 2001 May;31(5):1620-7
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Intracellular staining of normal human peripheral blood cells either unstimulated (orange historgam) or stimulated for 3 days with Anti-Human CD3 (Product # 16-0037-81) and Anti-Human CD28 (Product # 16-0289-81) (purple histogram) with Anti-Human BATF eFluor® 660 using the Foxp3/Transcription Factor Buffer Set (Product # 00-5523-00) and protocol. Cell in the lymphocyte gate were used for analysis.