Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00010538-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00010538-M01, RRID:AB_537097
- Product name
- Mouse Monoclonal BATF Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. BATF - basic leucine zipper transcription factor, ATF-like
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Basic leucine zipper transcription factor, ATF-like (BATF) regulates epigenetically and energetically effector CD8 T-cell differentiation via Sirt1 expression.
Kuroda S, Yamazaki M, Abe M, Sakimura K, Takayanagi H, Iwai Y
Proceedings of the National Academy of Sciences of the United States of America 2011 Sep 6;108(36):14885-9
Proceedings of the National Academy of Sciences of the United States of America 2011 Sep 6;108(36):14885-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BATF Antibody (8A12) [H00010538-M01] - Analysis of BATF expression in transfected 293T cell line by BATF monoclonal antibody (M01), clone 8A12.Lane 1: BATF transfected lysate(14.1 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BATF Antibody (8A12) [H00010538-M01] - BATF monoclonal antibody (M01), clone 8A12 Analysis of BATF expression in Hela S3 NE.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BATF Antibody (8A12) [H00010538-M01] - BATF monoclonal antibody (M01), clone 8A12. Analysis of BATF expression in PC-12.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: BATF Antibody (8A12) [H00010538-M01] - Detection limit for recombinant GST tagged BATF is approximately 1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: BATF Antibody (8A12) [H00010538-M01] - Analysis of BATF transfected lysate using anti-BATF monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with BATF MaxPab rabbit polyclonal antibody.