NBP2-59108

METAP1 antibody from Novus Biologicals

KIAA0094, MAP1A, MetAP1A

Western blot

Supportive data in Antibodypedia

Immunoprecipitation

Supportive data in Antibodypedia

Provider product page for NBP2-59108

Antibody Data

Product number

NBP2-59108

Provider

Novus Biologicals

Product name

Rabbit Polyclonal Methionine Aminopeptidase 1/METAP1 Antibody

Provider product page

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Storage

Store at 4C.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Methionine Aminopeptidase 1/METAP1 Antibody [NBP2-59108] - Detection of human and mouse METAP1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-METAP1 antibody NBP2-59108 used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Methionine Aminopeptidase 1/METAP1 Antibody [NBP2-59108] - Detection of human and mouse METAP1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-METAP1 antibody NBP2-59108 used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: Methionine Aminopeptidase 1/METAP1 Antibody [NBP2-59108] - Detection of human METAP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-METAP1 antibody NBP2-59108 used for IP at 20 ul per reaction. METAP1 was also immunoprecipitated by another rabbit anti-METAP1 antibody. For blotting immunoprecipitated METAP1, NBP2-59108 was used at 1:100. Detection: Chemiluminescence with an exposure time of 30 seconds.