PAB23810

antibody from Abnova Corporation

Targeting: POLR3E C37, FLJ10509, RPC5, SIN

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB23810

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB23810, RRID:AB_11123592

Product name

POLR3E polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant POLR3E.

Antigen sequence

FVMWKFTQSRWVVRKEVATVTKLCAEDVKDFLEHM
AVVRINKGWEFILPYDGEFIKKHPDVVQRQHMLWT
GIQAKLEKVYNLVKETMPKKPDAQ


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with POLR3E polyclonal antibody (Cat # PAB23810) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with POLR3E polyclonal antibody (Cat # PAB23810) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human stomach with POLR3E polyclonal antibody (Cat # PAB23810) shows strong nuclear positivity in glandular cells at 1:200-1:500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)