PA5-118175

antibody from Invitrogen Antibodies

Targeting: POLR3E C37, FLJ10509, RPC5, SIN

Western blot (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-118175

Provider

Invitrogen Antibodies

Product name

POLR3E Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of POLR3E in Lane A: 0.5 mg HeLa Whole Cell Lysate. Samples were treated with 60 μg of immunomagnetic Protein A/G beads, incubated with polyclonal antibody (Product # PA5-118175) with a dilution of 1:100 , followed by Goat Anti-Rabbit IgG (H+L)/HRP using a dilution of 1:10,000. Assay was performed under reducing conditions. Predicted band size: 80 kDa , Observed band size : 80 kDa .

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

POLR3E Immunoprecipitation using: Lane A: 0.5 mg HeLa Whole Cell Lysate 4 µL with POLR3E Polyclonal Antibody (Product # PA5-118175) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: POLR3E Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 80 kDa. Observed band size: 80 kDa.