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- Antibody Data
- Antigen structure
- References [18]
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- 10717-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#10717-1-AP, RRID:AB_2248272
- Product name
- HINT1 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated HINT1 antibody (Cat. #10717-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Identification and Validation of Novel Potential Pathogenesis and Biomarkers to Predict the Neurological Outcome after Cardiac Arrest.
Comparative Proteomic Analysis of HPV(+) Oropharyngeal Squamous Cell Carcinoma Recurrence.
A Chemical Strategy for Intracellular Arming of an Endogenous Broad-Spectrum Antiviral Nucleotide.
Methods to separate nuclear soluble fractions reflecting localizations in living cells.
Second messenger Ap(4)A polymerizes target protein HINT1 to transduce signals in FcεRI-activated mast cells.
The role of the Hint1 protein in the metabolism of phosphorothioate oligonucleotides drugs and prodrugs, and the release of H(2)S under cellular conditions.
Post-translational modification of HINT1 mediates activation of MITF transcriptional activity in human melanoma cells.
Interactions of cellular histidine triad nucleotide binding protein 1 with nucleosides 5'-O-monophosphorothioate and their derivatives - Implication for desulfuration process in the cell.
The tumor suppressor HINT1 regulates MITF and β-catenin transcriptional activity in melanoma cells.
Clinical significance of expression of Hint1 and potential epigenetic mechanism in gastric cancer.
Association of the histidine-triad nucleotide-binding protein-1 (HINT1) gene variants with nicotine dependence.
Mechanism of activation of PSI-7851 and its diastereoisomer PSI-7977.
Silencing of Hint1, a novel tumor suppressor gene, by promoter hypermethylation in hepatocellular carcinoma.
Novel breast cancer metastasis-associated proteins.
Histidine triad nucleotide-binding protein 1 up-regulates cellular levels of p27KIP1 by targeting ScfSKP2 ubiquitin ligase and Src.
Identifying apoptosis-evasion proteins/pathways in human hepatoma cells via induction of cellular hormesis by UV irradiation.
Proteomic analysis of low dose arsenic and ionizing radiation exposure on keratinocytes.
Distribution and expression of protein kinase C interactive protein (PKCI/HINT1) in mouse central nervous system (CNS).
Zhang Q, Zhang C, Liu C, Zhan H, Li B, Lu Y, Wei H, Cheng J, Li S, Wang C, Hu C, Liao X
Brain sciences 2022 Jul 15;12(7)
Brain sciences 2022 Jul 15;12(7)
Comparative Proteomic Analysis of HPV(+) Oropharyngeal Squamous Cell Carcinoma Recurrence.
Ho AS, Robinson A, Shon W, Laury A, Raedschelders K, Venkatraman V, Holewinski R, Zhang Y, Shiao SL, Chen MM, Mallen-St Clair J, Lin DC, Zumsteg ZS, Van Eyk JE
Journal of proteome research 2022 Jan 7;21(1):200-208
Journal of proteome research 2022 Jan 7;21(1):200-208
A Chemical Strategy for Intracellular Arming of an Endogenous Broad-Spectrum Antiviral Nucleotide.
Passow KT, Caldwell HS, Ngo KA, Arnold JJ, Antczak NM, Narayanan A, Jose J, Sturla SJ, Cameron CE, Ciota AT, Harki DA
Journal of medicinal chemistry 2021 Oct 28;64(20):15429-15439
Journal of medicinal chemistry 2021 Oct 28;64(20):15429-15439
Methods to separate nuclear soluble fractions reflecting localizations in living cells.
Ogawa Y, Imamoto N
iScience 2021 Dec 17;24(12):103503
iScience 2021 Dec 17;24(12):103503
Second messenger Ap(4)A polymerizes target protein HINT1 to transduce signals in FcεRI-activated mast cells.
Yu J, Liu Z, Liang Y, Luo F, Zhang J, Tian C, Motzik A, Zheng M, Kang J, Zhong G, Liu C, Fang P, Guo M, Razin E, Wang J
Nature communications 2019 Oct 11;10(1):4664
Nature communications 2019 Oct 11;10(1):4664
The role of the Hint1 protein in the metabolism of phosphorothioate oligonucleotides drugs and prodrugs, and the release of H(2)S under cellular conditions.
Krakowiak A, Piotrzkowska D, Kocoń-Rębowska B, Kaczmarek R, Maciaszek A
Biochemical pharmacology 2019 May;163:250-259
Biochemical pharmacology 2019 May;163:250-259
Post-translational modification of HINT1 mediates activation of MITF transcriptional activity in human melanoma cells.
Motzik A, Amir E, Erlich T, Wang J, Kim BG, Han JM, Kim JH, Nechushtan H, Guo M, Razin E, Tshori S
Oncogene 2017 Aug 17;36(33):4732-4738
Oncogene 2017 Aug 17;36(33):4732-4738
Interactions of cellular histidine triad nucleotide binding protein 1 with nucleosides 5'-O-monophosphorothioate and their derivatives - Implication for desulfuration process in the cell.
Krakowiak A, Pawłowska R, Kocoń-Rębowska B, Dolot R, Stec WJ
Biochimica et biophysica acta 2014 Dec;1840(12):3357-66
Biochimica et biophysica acta 2014 Dec;1840(12):3357-66
The tumor suppressor HINT1 regulates MITF and β-catenin transcriptional activity in melanoma cells.
Genovese G, Ghosh P, Li H, Rettino A, Sioletic S, Cittadini A, Sgambato A
Cell cycle (Georgetown, Tex.) 2012 Jun 1;11(11):2206-15
Cell cycle (Georgetown, Tex.) 2012 Jun 1;11(11):2206-15
Clinical significance of expression of Hint1 and potential epigenetic mechanism in gastric cancer.
Huang H, Wei X, Su X, Qiao F, Xu Z, Gu D, Fan H, Chen J
International journal of oncology 2011 Jun;38(6):1557-64
International journal of oncology 2011 Jun;38(6):1557-64
Association of the histidine-triad nucleotide-binding protein-1 (HINT1) gene variants with nicotine dependence.
Jackson KJ, Chen Q, Chen J, Aggen SH, Kendler KS, Chen X
The pharmacogenomics journal 2011 Aug;11(4):251-7
The pharmacogenomics journal 2011 Aug;11(4):251-7
Mechanism of activation of PSI-7851 and its diastereoisomer PSI-7977.
Murakami E, Tolstykh T, Bao H, Niu C, Steuer HM, Bao D, Chang W, Espiritu C, Bansal S, Lam AM, Otto MJ, Sofia MJ, Furman PA
The Journal of biological chemistry 2010 Nov 5;285(45):34337-47
The Journal of biological chemistry 2010 Nov 5;285(45):34337-47
Silencing of Hint1, a novel tumor suppressor gene, by promoter hypermethylation in hepatocellular carcinoma.
Zhang YJ, Li H, Wu HC, Shen J, Wang L, Yu MW, Lee PH, Bernard Weinstein I, Santella RM
Cancer letters 2009 Mar 18;275(2):277-84
Cancer letters 2009 Mar 18;275(2):277-84
Novel breast cancer metastasis-associated proteins.
Ho J, Kong JW, Choong LY, Loh MC, Toy W, Chong PK, Wong CH, Wong CY, Shah N, Lim YP
Journal of proteome research 2009 Feb;8(2):583-94
Journal of proteome research 2009 Feb;8(2):583-94
Histidine triad nucleotide-binding protein 1 up-regulates cellular levels of p27KIP1 by targeting ScfSKP2 ubiquitin ligase and Src.
Cen B, Li H, Weinstein IB
The Journal of biological chemistry 2009 Feb 20;284(8):5265-76
The Journal of biological chemistry 2009 Feb 20;284(8):5265-76
Identifying apoptosis-evasion proteins/pathways in human hepatoma cells via induction of cellular hormesis by UV irradiation.
Hsieh SY, Hsu CY, He JR, Liu CL, Lo SJ, Chen YC, Huang HY
Journal of proteome research 2009 Aug;8(8):3977-86
Journal of proteome research 2009 Aug;8(8):3977-86
Proteomic analysis of low dose arsenic and ionizing radiation exposure on keratinocytes.
Berglund SR, Santana AR, Li D, Rice RH, Rocke DM, Goldberg Z
Proteomics 2009 Apr;9(7):1925-38
Proteomics 2009 Apr;9(7):1925-38
Distribution and expression of protein kinase C interactive protein (PKCI/HINT1) in mouse central nervous system (CNS).
Liu Q, Puche AC, Wang JB
Neurochemical research 2008 Jul;33(7):1263-76
Neurochemical research 2008 Jul;33(7):1263-76
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 10717-1-AP(HINT1 antibody) at dilution of 1:400
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP Result of anti-HINT1 (IP:10717-1-AP, 3ug; Detection:10717-1-AP 1:500) with mouse brain tissue lysate 7500ug.
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The HINT1 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human HINT1. This antibody recognizes human,mouse,rat antigen. The HINT1 antibody has been validated for the following applications: ELISA, WB, IHC, IP analysis.
