H00003094-M05

antibody from Abnova Corporation

Targeting: HINT1 HINT, PKCI-1, PRKCNH1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00003094-M05

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00003094-M05, RRID:AB_1674847

Product name

HINT1 monoclonal antibody (M05), clone 2D7

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant HINT1.

Antigen sequence

MADEIAKAQVARPGGDTIFGKIIRKEIPAKIIFED
DRCLAFHDISPQAPTHFLVIPKKHISQISVAEDDD
ESLLGHLMIVGKKCAADLGLNKGYRMVVNEGSDGG
QSVYHVHLHVLGGRQMHWPPG


Isotype

IgG

Antibody clone number

2D7

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of HINT1 expression in transfected 293T cell line by HINT1 monoclonal antibody (M05), clone 2D7.Lane 1: HINT1 transfected lysate(13.8 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to HINT1 on HeLa cell . [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to HINT1 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol