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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- PAB3129 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3129, RRID:AB_1574737
- Product name
- ERAS polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of ERAS.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Human ERas gene has an upstream premature polyadenylation signal that results in a truncated, noncoding transcript.
Role of ERas in promoting tumour-like properties in mouse embryonic stem cells.
The human c-Ha-ras2 is a processed pseudogene inactivated by numerous base substitutions.
Kameda T, Thomson JA
Stem cells (Dayton, Ohio) 2005 Nov-Dec;23(10):1535-40
Stem cells (Dayton, Ohio) 2005 Nov-Dec;23(10):1535-40
Role of ERas in promoting tumour-like properties in mouse embryonic stem cells.
Takahashi K, Mitsui K, Yamanaka S
Nature 2003 May 29;423(6939):541-5
Nature 2003 May 29;423(6939):541-5
The human c-Ha-ras2 is a processed pseudogene inactivated by numerous base substitutions.
Miyoshi J, Kagimoto M, Soeda E, Sakaki Y
Nucleic acids research 1984 Feb 24;12(4):1821-8
Nucleic acids research 1984 Feb 24;12(4):1821-8
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of ERAS polyclonal antibody (Cat # PAB3129) in K-562 cell line lysates (35 ug/lane). ERAS (arrow) was detected using the purified polyclonal antibody.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of ERAS polyclonal antibody (Cat # PAB3129) in HeLa cells. 0.025 mg/mL primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human brain tissue reacted with ERAS polyclonal antibody (Cat # PAB3129) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HeLa cells using ERAS polyclonal antibody (Cat # PAB3129)(bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- Flow Cytometry
