Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [7]
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- Product number
- MA1-41247 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- APIP Monoclonal Antibody (19F461)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Suggested positive control: HeLa or 293, antigen standard for APIP (transient overexpression lysate).
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 19F461
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of APIP2 in A) recombinant protein andB) HeLa cell lysate using a APF1 Interacting Protein monoclonal antibody (Product # MA1-41247) at 2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of APIP in (A) recombinant protein and (B) HeLa cell lysate. Samples were incubated in APIP monoclonal antibody (Product # MA1-41247) using a dilution of 2 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of APIP in a section of normal prostate from human. Samples were incubated in APIP monoclonal antibody (Product # MA1-41247) using a dilution of 5 µg/mL. The tubuloalveolar glands in prostate section depicted APIP positivity in the cytoplasm and nuclei of the epithelial cells. The fibro-muscular stroma showed an overall weak staining with intense nuclear positivity in some cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of APIP in a section of normal human breast tissue. Samples were incubated in APIP monoclonal antibody (Product # MA1-41247) using a dilution of 5 µg/mL. The breasts ductal/acinar epithelial cells showed strong cytoplasmic as well as nuclear expression, whereas the myoepithelial cells and the intra-lobular connective tissue depicted very weak to negligible APIP positivity.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of APIP in formalin-fixed, paraffin-embedded adenocarcinoma of the breast. Samples were incubated in APIP monoclonal antibody (Product # MA1-41247) using a dilution of 5 µg/mL followed by peroxidase-conjugate and DAB chromogen. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of APIP in formalin-fixed, paraffin-embedded human stomach. Samples were incubated in APIP monoclonal antibody (Product # MA1-41247) using a dilution of 5 µg/mL followed by peroxidase-conjugate and DAB chromogen. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of APIP in human lung cancer tissue section. Samples were incubated in APIP monoclonal antibody (Product # MA1-41247) using a dilution of 5 µg/mL. The representative image shows a nuclear and cytoplasmic staining pattern of APIP expression in lung cancer cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of APIP in human renal cancer tissue section. Samples were incubated in APIP monoclonal antibody (Product # MA1-41247) using a dilution of 5 µg/mL. The representative image shows a nuclear and cytoplasmic staining pattern of APIP expression in cancer cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of APIP in formalin-fixed paraffin-embedded human kidney tissue. Samples were incubated in APIP monoclonal antibody (Product # MA1-41247) using a dilution of 5 µg/mL.