Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-29681 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RAC2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Molt-4, Raji.
- Concentration
- 1.43 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of THP1 (Lane 1), U937 (Lane 2), Jurkat (Lane 3), MCF7 (Lane 4), A549 (Lane 5), Caco2 (Lane 6) and HepG2 (Lane 7). The blot was probed with Anti-RAC2 Polyclonal Antibody (Product # PA5-29681, 1:3000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 21 kDa band corresponding to RAC2 was observed across all the cell lines tested except MCF7, A549, Caco2 and HepG2. Additional non-specific bands were also observed at 35 and 50 kDa across the cell lines.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RAC2 Polyclonal Antibody (Product # PA5-29681). Sample (30 µg of whole cell lysate). Lane A: Molt-4. 12% SDS PAGE. RAC2 Polyclonal Antibody (Product # PA5-29681) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using RAC2 (Product # PA5-29681) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RAC2 Polyclonal Antibody detects RAC2 protein at cytosol on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. RAC2 Polyclonal Antibody (Product # PA5-29681) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.