MA1-19209

antibody from Invitrogen Antibodies

Targeting: TUBA1C bcm948, MGC10851, MGC14580, TUBA6

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-19209

Provider

Invitrogen Antibodies

Product name

alpha Tubulin Monoclonal Antibody (TU-16)

Antibody type

Monoclonal

Antigen

Other

Description

This antibody reacts with alpha-tubulin of all tested species, under denaturing and non-denaturing conditions.

Antibody clone number

TU-16

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blotting analysis of human alpha-tubulin using mouse monoclonal antibody TU-16 on lysates of various cell lines and porcine brain under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2µg/mL of mouse anti-alpha-tubulin Monoclonal antibody (Product # MA1-19209) followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for alpha-tubulin at approximately 54kDa, nonspecific minor bands above 100 kDa do not interfere with specific signal.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry staining of alpha-tubulin in Hep-2 cells using mouse Monoclonal antibody TU-16 (diluted 1:400) (Product # MA1-19209), detected with GAM IgG-Alexa Fluor®488 (diluted 1:200; green).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry staining (paraffin sections) of alpha-tubulin in human stomach using mouse Monoclonal antibody TU-16 (diluted 1:400) (Product # MA1-19209), detected with GAM IgG-Alexa Fluor®488 (diluted 1:200; green).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry staining of human heart (paraffin sections) using anti-alpha tubulin (TU-16) Monoclonal antibody (Product # MA1-19209).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of alpha Tubulin using a monoclonal antibody (Product # MA1-19209).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of alpha-tubulin from HeLa and RAJI cell lysate by TU-16 Monoclonal antibody (Product # MA1-19209) and its detection by antibody TU-01. IgM heavy chain (76-92 kDa) and IgM light chain (25-30 kDa) indicated. MW of alpha tubulin is around 50 kDa. L = lysate; IPr = immunoprecipitate (reducing conditions).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of alpha-tubulin from HeLa and RAJI cell lysate by TU-16 Monoclonal antibody (Product # MA1-19209) and its detection by antibody TU-01. IgM heavy chain (76-92 kDa) and IgM light chain (25-30 kDa) indicated. MW of alpha tubulin is around 50 kDa. L = lysate; IPr = immunoprecipitate (reducing conditions).