12205-1-AP

antibody from Proteintech Group

Targeting: TRIM27 RFP, RNF76

Provider product page for 12205-1-AP

Western blot

ELISA

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [31]
Comments [0]
Validations
Western blot [1]
Immunohistochemistry [1]

Submit

Product number: 12205-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#12205-1-AP, RRID:AB_2256660
Product name: TRIM27 antibody
Antibody type: Polyclonal
Description: KD/KO validated TRIM27 antibody (Cat. #12205-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IHC, IP, WB, ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

TRIM27 protein structure - 12205-1-AP shown in red.

Submitted references TRIM27-controlled endothelium-derived exosomes play a central role in podocyte injury in diabetic kidney disease.

Tian Y, Liu Y, Feng X, Wu L, Song W, Zhao T, Liu J, Miao X, Ma H, Jia B, Kang L, Liu Q, Zhang W, Guo H, Yang L, Xu J, Liu S
Cell death discovery 2026 Mar 7;12(1)

TRIM27 promotes microglial M2 polarization and neuroprotection via TBK1-dependent autophagy in cerebral ischemia-reperfusion injury.

Xie D, Hu S, Long P, Huang J, Li X, Yuan Y, Liu H, Chen Y
International immunopharmacology 2026 Jun 15;179:116597

MYC-driven TRIM27 upregulation promotes prostate cancer progression by enhancing CANX ubiquitination and activating PI3K/AKT signaling.

Wang Z, Ning J, Xu L, Cheng F
Functional & integrative genomics 2026 Jan 20;26(1):29

Interaction of CSN4 to DDB1 regulates its stability and function in DNA damage signaling.

Choi SH, Cho K, Kim ES, Yoo HY
Cellular and molecular life sciences : CMLS 2026 Feb 13;83(1):111

Baicalin inhibits LPS-induced apoptosis and inflammation in WI- 38 cells by promoting FOXA2/TRIM27 Interaction: Implications for pediatric pneumonia mechanisms.

Gao L, Zeng X, Huang Y, Huang L
Naunyn-Schmiedeberg's archives of pharmacology 2025 Oct;398(10):14131-14142

Mitochondrial Genome-Encoded lncND5 Regulates Mitophagy in Hypoxic Pulmonary Artery Smooth Muscle Cell.

Bai J, He S, Wang X, Zhang L, Ma C, Gao D, Yuan H, Mei J, Guan X, Yu H, Wan K, Zhu D
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2025 May 31;39(10):e70618

Small-molecule allosteric activator of ubiquitin-specific protease 7 (USP7).

Jaen Maisonet I, Sharafi M, Korchak EJ, Salazar-Chaparro A, Bratt A, Parikh T, Varca AC, Shah B, Darnowski M, Chung M, Teh WP, Che J, Bezsonova I, Buhrlage SJ
bioRxiv : the preprint server for biology 2025 Mar 16;

SCAP Interacts with Trim27 to Promote Vascular Neointimal Hyperplasia by Regulating the Stability and Ubiquitination of IκBα.

Yao Y, Wang X, Xuan C, Li D, Huang W, Wei L, Ruan XZ, Li D, Chen Y
Aging and disease 2025 Aug 6;

Tripartite motif containing 27 alleviated septic liver injury via the HSPA8/JNK pathway in mice.

Yue Z, Yang J, Yu X, Shichen M, Feng Z, Zhong T, Guan J, Chang P, Liu Z
International immunopharmacology 2025 Aug 28;161:115031

HMGB1 Encapsulated in Podocyte-Derived Exosomes Plays a Central Role in Glomerular Endothelial Cell Injury in Lupus Nephritis by Regulating TRIM27 Expression.

Liu J, Zhao T, Cui H, Tian Y, Miao X, Xing L, Wang X, Huang J, Liu Q, Zhang W, Shi K, Liu Y, Jia B, Kang L, Tian Y, Yuan W, He S, Feng X, Liu S
Laboratory investigation; a journal of technical methods and pathology 2025 Apr;105(4):104096

O-GlcNAcylated RALY Contributes to Hepatocellular Carcinoma Cells Proliferation by Regulating USP22 mRNA Nuclear Export.

Liu S, Lv Q, Mao X, Dong H, Xu W, Du X, Jia W, Feng K, Zhang J, Zhang Y
International journal of biological sciences 2024;20(9):3675-3690

LTN1 promotes RLR degradation to inhibit immune response to RNA virus through the ESCRT pathway.

Qin F, Cai B, Wang P, Cao R, Zhang Y, Wen H, Zheng Y, Zhao W, Gao C, Liu B
Autophagy 2024 Jun;20(6):1270-1285

Trim27 aggravates airway inflammation and oxidative stress in asthmatic mice via potentiating the NLRP3 inflammasome.

Liu K, Gu Y, Gu S, Song L, Hua S, Li D, Tang M
International immunopharmacology 2024 Jun 15;134:112199

Proteomic profiling of centrosomes across multiple mammalian cell and tissue types by an affinity capture method.

Carden S, Vitiello E, Rosa E Silva I, Holder J, Quarantotti V, Kishore K, Roamio Franklin VN, D'Santos C, Ochi T, van Breugel M, Gergely F
Developmental cell 2023 Nov 6;58(21):2393-2410.e9

TRIM27 expression is associated with poor prognosis in sinonasal mucosal melanoma.

Kimura S, Suzuki M, Nakamaru Y, Kano S, Watanabe M, Honma A, Nakazono A, Tsushima N, Hatakeyama S, Homma A
Rhinology 2023 Jun 1;61(3):263-271

Drosophila as Model System to Study Ras-Mediated Oncogenesis: The Case of the Tensin Family of Proteins.

Martínez-Abarca Millán A, Soler Beatty J, Valencia Expósito A, Martín-Bermudo MD
Genes 2023 Jul 23;14(7)

MAGED4B Promotes Glioma Progression via Inactivation of the TNF-α-induced Apoptotic Pathway by Down-regulating TRIM27 Expression.

Liu C, Liu J, Shao J, Huang C, Dai X, Shen Y, Hou W, Shen Y, Yu Y
Neuroscience bulletin 2023 Feb;39(2):273-291

TRIM27 is an autophagy substrate facilitating mitochondria clustering and mitophagy via phosphorylated TBK1.

Garcia-Garcia J, Berge AKM, Overå KS, Larsen KB, Bhujabal Z, Brech A, Abudu YP, Lamark T, Johansen T, Sjøttem E
The FEBS journal 2023 Feb;290(4):1096-1116

SENP3 and USP7 regulate Polycomb-rixosome interactions and silencing functions.

Zhou H, Feng W, Yu J, Shafiq TA, Paulo JA, Zhang J, Luo Z, Gygi SP, Moazed D
Cell reports 2023 Apr 25;42(4):112339

TRIM27 regulates the expression of PDCD4 by the ubiquitin‑proteasome pathway in ovarian and endometrial cancer cells.

Yu H, Wan L, Tang Z, Yao C, Zhang D, Jiang M, Wang C, Liu Y, Xue C, Wang X, Shi Y, Zhang L, Wang X, Wei Z
Oncology reports 2022 Jul;48(1)

Long non-coding RNA TRIM52-AS1 sponges microRNA-577 to facilitate diffuse large B cell lymphoma progression via increasing TRIM52 expression.

Zhao F, Li S, Liu J, Wang J, Yang B
Human cell 2022 Jul;35(4):1234-1247

TRIM27-mediated ubiquitination of PPARγ promotes glutamate-induced cell apoptosis and inflammation.

Hao Y, Bai S, Peng J, Hong R, Ding J, Li Z, Guan Y
Experimental cell research 2021 Mar 1;400(1):112437

NT5DC2 promotes leiomyosarcoma tumour cell growth via stabilizing unpalmitoylated TEAD4 and generating a positive feedback loop.

Hu B, Zhou S, Hu X, Zhang H, Lan X, Li M, Wang Y, Hu Q
Journal of cellular and molecular medicine 2021 Jul;25(13):5976-5987

TRIM28 and TRIM27 are required for expressions of PDGFRβ and contractile phenotypic genes by vascular smooth muscle cells.

Wang Y, Hao Y, Zhao Y, Huang Y, Lai D, Du T, Wan X, Zhu Y, Liu Z, Wang Y, Wang N, Zhang P
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 May;34(5):6271-6283

The testis protein ZNF165 is a SMAD3 cofactor that coordinates oncogenic TGFβ signaling in triple-negative breast cancer.

Gibbs ZA, Reza LC, Cheng CC, Westcott JM, McGlynn K, Whitehurst AW
eLife 2020 Jun 9;9

TRIM27 Functions as a Novel Oncogene in Non-Triple-Negative Breast Cancer by Blocking Cellular Senescence through p21 Ubiquitination.

Xing L, Tang X, Wu K, Huang X, Yi Y, Huan J
Molecular therapy. Nucleic acids 2020 Dec 4;22:910-923

TRIM27 promotes the development of esophagus cancer via regulating PTEN/AKT signaling pathway.

Ma L, Yao N, Chen P, Zhuang Z
Cancer cell international 2019;19:283

Knockdown of TRIM27 expression suppresses the dysfunction of mesangial cells in lupus nephritis by FoxO1 pathway.

Liu J, Feng X, Tian Y, Wang K, Gao F, Yang L, Li H, Tian Y, Yang R, Zhao L, Miao X, Huang J, Liu Q, Zhang W, Li Y, Wang C, Duan H, Liu S
Journal of cellular physiology 2019 Jul;234(7):11555-11566

Trim27 confers myeloid hematopoiesis competitiveness by up-regulating myeloid master genes.

Wang T, Xia C, Dong Y, Chen X, Wang J, Du J
Journal of leukocyte biology 2018 Oct;104(4):799-809

Downregulation of TRIM27 expression inhibits the proliferation of ovarian cancer cells in vitro and in vivo.

Ma Y, Wei Z, Bast RC Jr, Wang Z, Li Y, Gao M, Liu Y, Wang X, Guo C, Zhang L, Wang X
Laboratory investigation; a journal of technical methods and pathology 2016 Jan;96(1):37-48

Nicotine Induced Murine Spermatozoa Apoptosis via Up-Regulation of Deubiquitinated RIP1 by Trim27 Promoter Hypomethylation.

Nie D, Zhang D, Dai J, Zhang M, Zhao X, Xu W, Chen Z, Wang L, Wang Z, Qiao Z
Biology of reproduction 2016 Feb;94(2):31

No comments: Submit comment

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Jurkat cells were subjected to SDS PAGE followed by western blot with 12205-1-AP(TRIM27 antibody) at dilution of 1:1000
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The TRIM27 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human TRIM27. This antibody recognizes human, mouse, rat antigen. The TRIM27 antibody has been validated for the following applications: ELISA, WB, IHC analysis.
Appendix: 12205-1-AP.pdf

: Show more