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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-58217 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PDSS1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MGKPTSADLK LGLATGPVLF ACQQFPEMNA MIMRRFSLPG DVDRARQYVL QSDGVQQTTY LAQQYCHEAI REISKLRPSP ERDALIQLSE IVLT Highest antigen sequence identity to the following orthologs: Mouse - 94%, Rat - 94%.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Decaprenyl Diphosphate Synthase Subunit 1 (PDSS1): A Potential Prognostic Biomarker and Immunotherapy-Target for Hepatocellular Carcinoma.
Yang Y, Li J, Tang M, Nie B, Huang W
Cancer management and research 2022;14:1627-1639
Cancer management and research 2022;14:1627-1639
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PDSS1 in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using a PDSS1 Polyclonal Antibody (Product # PA5-58217).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PDSS1 in Lane 1: Marker (kDa) 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp; Lane 4: Human plasma (IgG/HSA depleted); Lane 5: Human liver tissue. Samples were probed using a PDSS1 Polyclonal Antibody (Product # PA5-58217).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of PDSS1 in human colon using a PDSS1 Polyclonal Antibody (Product # PA5-58217) shows strong cytoplasmic positivity in glandular cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- The levels of PDSS1 expression in human tumors. (The mRNA expression of PDSS1 in different types of human tumors was analyzed by Tumor Immune Estimation Resource (TIMER) ( A ) and UALCAN ( B ). There are representative immunohistochemistry staining images with a PDSS1 antibody in HCC tumor tissues and adjacent tissues in our cohort ( C ). High expression tumors: Bladder urothelial carcinoma (BLCA); Breast invasive carcinoma (BRCA); Cervical squamous cell carcinoma and endocervical adenocarcinoma (CESC); Liver hepatocellular carcinoma (LIHC);Colon adenocarcinoma (COAD); Esophageal carcinoma (ESCA); Glioblastoma multiforme (GBM); Brain Lower Grade Glioma (LGG); Head and neck squamous cell carcinoma (HNSC);Head and neck squamous cell carcinoma-HPV neg (HNSC- HPV neg); Liver hepatocellular carcinoma (LIHC);Lung adenocarcinoma (LUAD); Lung squamous cell carcinoma (LUSC); Prostate adenocarcinoma (PRAD); Stomach adenocarcinoma (STAD); Uterine Corpus Endometrial Carcinoma (UCEC). Low expression tumors: Kidney chromophobe (KICH); Thyroid carcinoma (THCA). No difference tumors: Breast invasive carcinoma-Basal (BRCA-Basal); Breast invasive carcinoma-Her2 (BRCA- Her2); Breast invasive carcinoma-Luminal (BRCA- Luminal); Kidney renal clear cell carcinoma (KIRC); Kidney renal papillary cell carcinoma (KIRP); Pancreatic adenocarcinoma (PAAD); Pheochromocytoma and Paraganglioma (PCPG); Rectum adenocarcinoma (READ). *Indicates P- value
