Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- MAB21861 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Carbonic Anhydrase IV/CA4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Carbonic Anhydrase IV/CA4 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human CA1, -2, -3, -5a, -5b, -7, 8, -9, or -10 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Connective Tissue Growth Factor Promotes Efficient Generation of Human Induced Pluripotent Stem Cell-Derived Choroidal Endothelium.
Songstad AE, Worthington KS, Chirco KR, Giacalone JC, Whitmore SS, Anfinson KR, Ochoa D, Cranston CM, Riker MJ, Neiman M, Stone EM, Mullins RF, Tucker BA
Stem cells translational medicine 2017 Jun;6(6):1533-1546
Stem cells translational medicine 2017 Jun;6(6):1533-1546
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Detection of Human Carbonic Anhydrase IV/CA4 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and human lung tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human Carbonic Anhydrase IV/CA4 Monoclonal Antibody (Catalog # MAB21861) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Carbonic Anhydrase IV/CA4 at approximately 35 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.