Antibody data
- Antibody Data
- Antigen structure
- References [12]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AF2414 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Carbonic Anhydrase IV/CA4 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse Carbonic Anhydrase IV/CA4 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human CA4 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q64444
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Bortezomib alters sour taste sensitivity in mice.
Transcriptome analyses of taste organoids reveal multiple pathways involved in taste cell generation.
The cellular mechanism for water detection in the mammalian taste system.
Rewiring the taste system.
Bcl11b/Ctip2 is required for development of lingual papillae in mice.
Normal Fertility Requires the Expression of Carbonic Anhydrases II and IV in Sperm.
Expression patterns and subcellular localization of carbonic anhydrases are developmentally regulated during tooth formation.
Carbonic anhydrase IV is expressed on IL-5-activated murine eosinophils.
Induction of ectopic taste buds by SHH reveals the competency and plasticity of adult lingual epithelium.
Defects in the peripheral taste structure and function in the MRL/lpr mouse model of autoimmune disease.
CO2 chemosensing in rat oesophagus.
Carbonic anhydrases and mucosal vanilloid receptors help mediate the hyperemic response to luminal CO2 in rat duodenum.
Ohishi A, Nishida K, Miyamoto K, Imai M, Nakanishi R, Kobayashi K, Hayashi A, Nagasawa K
Toxicology reports 2017;4:172-180
Toxicology reports 2017;4:172-180
Transcriptome analyses of taste organoids reveal multiple pathways involved in taste cell generation.
Ren W, Aihara E, Lei W, Gheewala N, Uchiyama H, Margolskee RF, Iwatsuki K, Jiang P
Scientific reports 2017 Jun 21;7(1):4004
Scientific reports 2017 Jun 21;7(1):4004
The cellular mechanism for water detection in the mammalian taste system.
Zocchi D, Wennemuth G, Oka Y
Nature neuroscience 2017 Jul;20(7):927-933
Nature neuroscience 2017 Jul;20(7):927-933
Rewiring the taste system.
Lee H, Macpherson LJ, Parada CA, Zuker CS, Ryba NJP
Nature 2017 Aug 17;548(7667):330-333
Nature 2017 Aug 17;548(7667):330-333
Bcl11b/Ctip2 is required for development of lingual papillae in mice.
Nishiguchi Y, Ohmoto M, Koki J, Enomoto T, Kominami R, Matsumoto I, Hirota J
Developmental biology 2016 Aug 1;416(1):98-110
Developmental biology 2016 Aug 1;416(1):98-110
Normal Fertility Requires the Expression of Carbonic Anhydrases II and IV in Sperm.
Wandernoth PM, Mannowetz N, Szczyrba J, Grannemann L, Wolf A, Becker HM, Sly WS, Wennemuth G
The Journal of biological chemistry 2015 Dec 4;290(49):29202-16
The Journal of biological chemistry 2015 Dec 4;290(49):29202-16
Expression patterns and subcellular localization of carbonic anhydrases are developmentally regulated during tooth formation.
Reibring CG, El Shahawy M, Hallberg K, Kannius-Janson M, Nilsson J, Parkkila S, Sly WS, Waheed A, Linde A, Gritli-Linde A
PloS one 2014;9(5):e96007
PloS one 2014;9(5):e96007
Carbonic anhydrase IV is expressed on IL-5-activated murine eosinophils.
Wen T, Mingler MK, Wahl B, Khorki ME, Pabst O, Zimmermann N, Rothenberg ME
Journal of immunology (Baltimore, Md. : 1950) 2014 Jun 15;192(12):5481-9
Journal of immunology (Baltimore, Md. : 1950) 2014 Jun 15;192(12):5481-9
Induction of ectopic taste buds by SHH reveals the competency and plasticity of adult lingual epithelium.
Castillo D, Seidel K, Salcedo E, Ahn C, de Sauvage FJ, Klein OD, Barlow LA
Development (Cambridge, England) 2014 Aug;141(15):2993-3002
Development (Cambridge, England) 2014 Aug;141(15):2993-3002
Defects in the peripheral taste structure and function in the MRL/lpr mouse model of autoimmune disease.
Kim A, Feng P, Ohkuri T, Sauers D, Cohn ZJ, Chai J, Nelson T, Bachmanov AA, Huang L, Wang H
PloS one 2012;7(4):e35588
PloS one 2012;7(4):e35588
CO2 chemosensing in rat oesophagus.
Akiba Y, Mizumori M, Kuo M, Ham M, Guth PH, Engel E, Kaunitz JD
Gut 2008 Dec;57(12):1654-64
Gut 2008 Dec;57(12):1654-64
Carbonic anhydrases and mucosal vanilloid receptors help mediate the hyperemic response to luminal CO2 in rat duodenum.
Akiba Y, Ghayouri S, Takeuchi T, Mizumori M, Guth PH, Engel E, Swenson ER, Kaunitz JD
Gastroenterology 2006 Jul;131(1):142-52
Gastroenterology 2006 Jul;131(1):142-52
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse Carbonic Anhydrase IV/CA4 by Western Blot. Western blot shows lysates of mouse kidney tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse Carbonic Anhydrase IV/CA4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2414) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Carbonic Anhydrase IV/CA4 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse Carbonic Anhydrase IV/CA4 by Simple WesternTM. Simple Western lane view shows lysates of mouse lung tissue and mouse kidney tissue, loaded at 0.2 mg/mL. A specific band was detected for Carbonic Anhydrase IV/CA4 at approximately 51-52 kDa (as indicated) using 12.5 µg/mL of Goat Anti-Mouse Carbonic Anhydrase IV/CA4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2414) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Carbonic Anhydrase IV/CA4 in Mouse Kidney. Carbonic Anhydrase IV/CA4 was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse Carbonic Anhydrase IV/CA4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2414) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.