MA5-24028

antibody from Invitrogen Antibodies

Targeting: CA4 CAIV, Car4, RP17

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-24028

Provider

Invitrogen Antibodies

Product name

Carbonic Anhydrase IV Monoclonal Antibody (310413)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

In direct ELISAs and Western blots, less than 10% cross-reactivity with recombinant human (rh) CA1, rhCA2, and recombinant mouse CA4 is observed. This antibody does not cross-react with rhCA3, 8, 9, 10, 12, 13, or 14.

Antibody clone number

310413

Concentration

0.5 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis from lysates of Jurkat human acute T cell leukemia cell line and human lung tissue. PVDF membrane was probed with 2 µg/mL of mouse Anti-human Carbonic Anhydrase IV/CA4 Monoclonal Antibody (Product # MA5-24028) followed by HRP-conjugated Anti-mouse IgG Secondary Antibody. A specific band was detected for Carbonic Anhydrase IV/CA4 at approximately XXX kDa (as indicated). This experiment was conducted under non-reducing conditions.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Carbonic Anhydrase IV in Jurkat human acute T cell leukemia cell line and human lung tissue. Samples were incubated in Carbonic Anhydrase IV monoclonal antibody (Product # MA5-24028) using a dilution of 2 µg/mL followed by a HRP-conjugated Anti-Mouse IgG secondary antibody. A specific band was detected for Carbonic Anhydrase IV/CA4 at approximately 35 kDa (as indicated). This experiment was conducted under non-reducing conditions.