Antibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
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Validation data
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- Product number
- PA5-47266 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Carbonic Anhydrase IV Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant mouse CA4 is observed and less than 5% cross-reactivity with recombinant human CA1, 2, 3, 8, 9, 10, 12, and 13 is observed. Reconstitute at 0.2 mg/mL in sterile PBS.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Carbonic Anhydrase IV in human brain (cerebellum) tissue, human lung tisssue, and A549 human lung carcinoma cell line. Samples were incubated in Carbonic Anhydrase IV polyclonal antibody (Product # PA5-47266) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. A specific band was detected for ICAM‚5 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Carbonic Anhydrase IV in 0.2 mg/mL lysates of human lung tissue and human kidney tissue. Samples were incubated in Carbonic Anhydrase IV polyclonal antibody (Product # PA5-47266) using a dilution of 10 µg/mL followed by HRP-conjugated Anti-Goat IgG at a dilution of 1:50. A specific band was detected for Carbonic Anhydrase IV/CA4 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.