TA501935

antibody from OriGene

Targeting: GSS

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA501935

Provider

OriGene

Proper citation

OriGene Cat#TA501935, RRID:AB_11127023

Product name

GSS (Glutathione Synthetase) mouse monoclonal antibody, clone OTI2G3 (formerly 2G3)

Antibody type

Monoclonal

Description

GSS (Glutathione Synthetase) mouse monoclonal antibody, clone OTI2G3 (formerly 2G3)

Reactivity

Canine

Host

Mouse

Conjugate

Unconjugated

Epitope

GSS

Isotype

IgG

Antibody clone number

OTI2G3

Vial size

100 µl

Concentration

0.6 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GSS monoclonal antibody.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GSS (RC203174, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GSS.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-GSS monoclonal antibody.(1:200)

Validation comment

WB

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC203174 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-GSS antibody(TA501935), and then analyzed by flow cytometry.

Validation comment

FC