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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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Validation data
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- Product number
- PA5-116970 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Tartrate Resistant Acid Phosphatase Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Samples: BT-474, Jurkat Immunogen sequence: TGVQDINDKR FQETFEDVFS DRSLRKVPWY VLAGNHDHLG NVSAQIAYSK ISKRWNFPSP FYRLHFKIPQ TNVSVAIFML DTVTLCGNSD DFLSQQPERP RDVKLARTQL SWLKKQLAAA REDYVLVAGH YPVWSIAEHG PTHCLVKQLR PLLATYGVTA YLCGHDHNLQ YLQDENGVGY VLSGAGNFMD PSKRHQRKVP NGYLRFHYGT EDSLGGFAYV EISSKEMTVT YIEASGKSLF KTRLPRRARP
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.62 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Epigallocatechin-3-gallate inhibits osteoclastic differentiation by modulating mitophagy and mitochondrial functions.
Estrogen Receptor 1 (ESR1) and the Wnt/β-Catenin Pathway Mediate the Effect of the Coumarin Derivative Umbelliferon on Bone Mineralization.
Sarkar J, Das M, Howlader MSI, Prateeksha P, Barthels D, Das H
Cell death & disease 2022 Oct 28;13(10):908
Cell death & disease 2022 Oct 28;13(10):908
Estrogen Receptor 1 (ESR1) and the Wnt/β-Catenin Pathway Mediate the Effect of the Coumarin Derivative Umbelliferon on Bone Mineralization.
Pelusi L, Mandatori D, Di Pietrantonio N, Del Pizzo F, Di Tomo P, Di Pietro N, Buda R, Genovese S, Epifano F, Pandolfi A, Fiorito S, Pipino C
Nutrients 2022 Aug 5;14(15)
Nutrients 2022 Aug 5;14(15)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of mouse kidney using Tartrate Resistant Acid Phosphatase Polyclonal Antibody (Product # PA5-116970) at a 1:1,000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Tartrate Resistant Acid Phosphatase using Tartrate Resistant Acid Phosphatase Polyclonal Antibody (Product # PA5-116970) at a 1:200 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis of Tartrate Resistant Acid Phosphatase in U2OS cells using Tartrate Resistant Acid Phosphatase Polyclonal Antibody (Product # PA5-116970) at a dilution of 1:100. Blue: DAPI for nuclear staining.
