A01715-1

antibody from Boster Biological Technology

Targeting: MTTP ABL, MTP

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

A01715-1

Provider

Boster Biological Technology

Product name

Anti-MTTP Antibody Picoband™

Antibody type

Polyclonal

Description

Rabbit IgG polyclonal antibody for MTTP detection. Tested with WB, IHC-P, ICC/IF, FCM, Direct ELISA in Human;Monkey;Rat.

Reactivity

Human, Rat, Simian

Host

Rabbit

Vial size

100μg/vial

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

References

Submitted references

Lipodystrophic gene Agpat2 deficiency aggravates hyperlipidemia and atherosclerosis in Ldlr(-/-) mice.

Peng K, Chen X, Pei K, Wang X, Ma X, Liang C, Dong Q, Liu Z, Han M, Liu G, Yang H, Zheng M, Liu G, Gao M
Biochimica et biophysica acta. Molecular basis of disease 2024 Jan;1870(1):166850

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Metabolic characteristics of voriconazole - Induced liver injury in rats.

Du Q, Teng M, Yang L, Meng C, Qiu Y, Wang C, Chen J, Wang T, Chen S, Luo Y, Sun J, Dong Y
Chemico-biological interactions 2023 Sep 25;383:110693

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of MTTP using anti-MTTP antibody (A01715-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEPG2 whole cell lysates, Lane 2: monkey liver tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: rat small intestine tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTTP antigen affinity purified polyclonal antibody (Catalog # A01715-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MTTP at approximately 99KD. The expected band size for MTTP is at 99KD.

Additional image