PA5-24865

antibody from Invitrogen Antibodies

Targeting: MFAP4

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-24865

Provider

Invitrogen Antibodies

Product name

MFAP4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with bovine based on sequence homology.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

TNF antagonist sensitizes synovial fibroblasts to ferroptotic cell death in collagen-induced arthritis mouse models.

Wu J, Feng Z, Chen L, Li Y, Bian H, Geng J, Zheng ZH, Fu X, Pei Z, Qin Y, Yang L, Zhao Y, Wang K, Chen R, He Q, Nan G, Jiang X, Chen ZN, Zhu P
Nature communications 2022 Feb 3;13(1):676

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of MFAP4 in formalin fixed and paraffin embedded human lung carcinoma. Samples were incubated with MFAP4 polyclonal antibody (Product # PA5-24865) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of MCF-7 cells using a MFAP4 polyclonal antibody (Product # PA5-24865) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 3 Single-cell RNA sequencing reveals fibroblast subsets with distinct ferroptosis sensitivity. a tSNE plot displaying 4735 CD45- cells from 3 STIA mice separated into 7 major cell types, including fibroblasts (9 clusters), vasculature (2 clusters), pericytes (1 cluster), chondrocytes (1 cluster), muscle cells (1 cluster), osteoblasts (2 clusters), and erythrocytes (2 clusters). b Heatmap showing the expression of high-risk ferroptosis genes in fibroblast clusters in the inflamed STIA joints. c Predicted ferroptosis-sensitive and ferroptosis-resistant fibroblast subsets based on ferroptosis-related genes listed in ( b ). d Expression of identified conserved marker genes in the ferroptosis-sensitive and ferroptosis-resistant fibroblast subsets. e Left, representative fluorescent multiplex IHC staining and quantification of inflamed joints of CIA mice labeled with anti-Mfap4 (red), anti-Sparcl1 (purple), anti-FAPalpha (green), and DAPI (blue). Mfap4 low , n = 9905 cells from 10 inflamed joints. Mfap4 high , n = 3336 cells from 10 inflamed joints. Right, representative fluorescent multiplex IHC staining (top panel) and quantification (bottom panel) of hyperplastic synovium of RA patients labeled with anti-Mfap4 (green), anti-Sparcl1 (red), anti-FAPalpha (orange), and DAPI (blue). Mfap4 low , n = 8222 cells from 10 independent human synovium samples. Mfap4 high , n = 6730 cells from 10 independent human synovium samples. Scale bars, 200 mum. **** P < 0.0001; two-tailed t -tes