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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- AF6120 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal Semaphorin 4C Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse and rat Semaphorin 4C in Western blots. In direct ELISAs, less than 20% cross-reactivity with recombinant human Semaphorin 4C and less than 5% cross-reactivity with recombinant mouse (rm) Semaphorin 4B, rmSemaphorin 4D, and rmSemaphorin 4G is observed.
- Reactivity
- Mouse, Rat
- Host
- Sheep
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Plexin B2 and Semaphorin 4C Guide T Cell Recruitment and Function in the Germinal Center.
Semaphorin 4C Protects against Allergic Inflammation: Requirement of Regulatory CD138+ Plasma Cells.
Yan H, Wu L, Shih C, Hou S, Shi J, Mao T, Chen W, Melvin B, Rigby RJ, Chen Y, Jiang H, Friedel RH, Vinuesa CG, Qi H
Cell reports 2017 May 2;19(5):995-1007
Cell reports 2017 May 2;19(5):995-1007
Semaphorin 4C Protects against Allergic Inflammation: Requirement of Regulatory CD138+ Plasma Cells.
Xue D, Kaufman GN, Dembele M, Beland M, Massoud AH, Mindt BC, Fiter R, Fixman ED, Martin JG, Friedel RH, Divangahi M, Fritz JH, Mazer BD
Journal of immunology (Baltimore, Md. : 1950) 2017 Jan 1;198(1):71-81
Journal of immunology (Baltimore, Md. : 1950) 2017 Jan 1;198(1):71-81
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Mouse and Rat Semaphorin 4C by Simple WesternTM. Detection of Mouse and Rat Semaphorin 4C by Simple WesternTM.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Mouse Semaphorin 4C by Western Blot (Non-Reducing). Detection of Mouse Semaphorin 4C by Western Blot (Non-Reducing).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Semaphorin 4C in Mouse Embryo. Semaphorin 4C in Mouse Embryo.
