Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AF6125 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal Semaphorin 4C Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Semaphorin 4C in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant mouse (rm) Sema4C is observed and less than 5% cross-reactivity with recombinant human (rh) Sema4G, rhSema4D, rhSema4B and rhSema4A is observed.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references SEMA4C is a novel target to limit osteosarcoma growth, progression, and metastasis.
A systems biology approach to characterize the regulatory networks leading to trabectedin resistance in an in vitro model of myxoid liposarcoma.
Smeester BA, Slipek NJ, Pomeroy EJ, Bomberger HE, Shamsan GA, Peterson JJ, Crosby MR, Draper GM, Becklin KL, Rahrmann EP, McCarthy JB, Odde DJ, Wood DK, Largaespada DA, Moriarity BS
Oncogene 2020 Jan;39(5):1049-1062
Oncogene 2020 Jan;39(5):1049-1062
A systems biology approach to characterize the regulatory networks leading to trabectedin resistance in an in vitro model of myxoid liposarcoma.
Uboldi S, Calura E, Beltrame L, Fuso Nerini I, Marchini S, Cavalieri D, Erba E, Chiorino G, Ostano P, D'Angelo D, D'Incalci M, Romualdi C
PloS one 2012;7(4):e35423
PloS one 2012;7(4):e35423
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Semaphorin 4C by Simple WesternTM. Detection of Human Semaphorin 4C by Simple WesternTM.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Semaphorin 4C by Western Blot. Detection of Human Semaphorin 4C by Western Blot.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Semaphorin 4C in Human Brain. Semaphorin 4C in Human Brain.