LS-C150248

antibody from LSBio

Targeting: SEMA4C Semacl1, Semaf, SEMAI

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C150248

Provider

LSBio

Product name

Anti-SEMA4C / Semaphorin 4C Antibody (aa22-664) LS-C150248

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Mouse

Host

Sheep

Isotype

IgG

Vial size

25µg

Storage

Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Semaphorin 4C in Rat Cortical Stem Cells. Semaphorin 4C was detected in immersion fixed rat cortical stem cells using Sheep Anti-Mouse Semaphorin 4C Antigen Affinity-purified Polyclonal Antibody at 10 ug/ml for 3 hours at room temperature. Cells were stained using NorthernLights 557-conjugated Anti-Sheep IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Semaphorin 4C in Mouse Embryo. Semaphorin 4C was detected in perfusion fixed frozen sections of mouse embryo (13 d. p. c.) using Sheep Anti-Mouse Semaphorin 4C Antigen Affinity-purified Polyclonal Antibody at 1.7 ug/ml overnight at 4°C. Tissue was stained using Anti-Sheep HRP-DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal processes of the developing cerebellum.