PA5-47814

antibody from Invitrogen Antibodies

Targeting: SEMA4C Semacl1, Semaf, SEMAI

Antibody data

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Validation data

Product number: PA5-47814 - Provider product page
Provider: Invitrogen Antibodies
Product name: SEMA4C Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant full-length protein
Description: In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse (rm) Sema4C is observed, and approximately 10% cross-reactivity with recombinant human (rh) Sema4G is observed, and less than 5% cross-reactivity with rhSema3B and rmSema3F is observed. Reconstitute at 0.2 mg/mL in sterile PBS.
Reactivity: Human
Host: Sheep
Isotype: IgG
Vial size: 100 µg
Concentration: 0.2 mg/mL
Storage: -20° C, Avoid Freeze/Thaw Cycles

SEMA4C protein structure - PA5-47814 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis from lysates of SW13 human adrenal cortex adenocarcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-human Semaphorin 4C Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47814) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. A specific band was detected for Semaphorin 4C at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis from lysates of SW13 human adrenal cortex adenocarcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-human Semaphorin 4C Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47814) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. A specific band was detected for Semaphorin 4C at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of SEMA4C in SW13 human adrenal cortex adenocarcinoma cell line. Samples were incubated in SEMA4C polyclonal antibody (Product # PA5-47814) using a dilution of 0.5 µg/mL followed by a HRP-conjugated Anti-Sheep IgG secondary antibody. A specific band was detected for Semaphorin 4C at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of SEMA4C in 0.2 mg/mL lysates of SW13 human adrenal cortex adenocarcinoma cell line. Samples were incubated in SEMA4C polyclonal antibody (Product # PA5-47814) using a dilution of 25 µg/mL followed by HRP-conjugated Anti-Sheep IgG at a dilution of 0.0763888888888889. A specific band was detected for Semaphorin 4C at approximately 121 kDa (as indicated). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.