Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 12-3059-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD305 (LAIR1) Monoclonal Antibody (NKTA255), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This NKTA255 monoclonal antibody reacts with human CD305, which is also known as leukocyte-associated Ig-like receptor (LAIR) 1. CD305 is a type I transmembrane glycoprotein that possesses an extracellular Ig-like domain and two intracellular ITIMs. CD305 is expressed on nearly all immune cells, including T, NK, and B cells, as well as monocytes, dendritic cells, eosinophils, basophils, and mast cells. In humans, this molecule is also expressed on CD34+ hematopoietic progenitor cells and thymocytes. Several isoforms of CD305 exist due to alternative splicing, and their expression may differ between cell types. As an inhibitory receptor, CD305 blocks cytokine-mediated signaling, cell proliferation, and apoptosis. Studies have also identified collagens as the ligands for CD305. The NKTA255 antibody has been reported to inhibit NK cytolytic activity. Applications Reported: This NKTA255 antibody has been reported for use in flow cytometric analysis. Applications Tested: This NKTA255 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- NKTA255
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Expression profiles of novel cell surface molecules on B-cell subsets and plasma cells as analyzed by flow cytometry.
The inhibitory collagen receptor LAIR-1 (CD305).
The Eighth Human Leucocyte Differentiation Antigen (HLDA8) Workshop: natural killer cell section report.
p40, a novel surface molecule involved in the regulation of the non-major histocompatibility complex-restricted cytolytic activity in humans.
Llinàs L, Lázaro A, de Salort J, Matesanz-Isabel J, Sintes J, Engel P
Immunology letters 2011 Jan 30;134(2):113-21
Immunology letters 2011 Jan 30;134(2):113-21
The inhibitory collagen receptor LAIR-1 (CD305).
Meyaard L
Journal of leukocyte biology 2008 Apr;83(4):799-803
Journal of leukocyte biology 2008 Apr;83(4):799-803
The Eighth Human Leucocyte Differentiation Antigen (HLDA8) Workshop: natural killer cell section report.
Warren HS
Cellular immunology 2005 Jul-Aug;236(1-2):17-20
Cellular immunology 2005 Jul-Aug;236(1-2):17-20
p40, a novel surface molecule involved in the regulation of the non-major histocompatibility complex-restricted cytolytic activity in humans.
Poggi A, Pella N, Morelli L, Spada F, Revello V, Sivori S, Augugliaro R, Moretta L, Moretta A
European journal of immunology 1995 Feb;25(2):369-76
European journal of immunology 1995 Feb;25(2):369-76
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD56 (NCAM) APC (Product # 17-0567-42) and Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (left) or Anti-Human CD305 (LAIR1) PE (right). Cells in the lymphocyte gate were used for analysis.
- Conjugate
- Yellow dye