Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- AF9055-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human SEC13 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human SEC13 in direct ELISA and Western blots.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
P55735
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human SEC13 by Western Blot. Western blot shows lysates of human kidney tissue and human lung tissue. PVDF membrane was probed with 0.05 µg/mL of Rabbit Anti-Human SEC13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF9055) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for SEC13 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human SEC13 by Simple WesternTM. Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for SEC13 at approximately 42 kDa (as indicated) using 0.5 µg/mL of Rabbit Anti-Human SEC13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF9055). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- SEC13 in HeLa Human Cell Line. SEC13 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human SEC13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF9055) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.