PA5-21339

antibody from Invitrogen Antibodies

Targeting: SEC13 D3S1231E, npp-20, SEC13L1, SEC13R

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-21339

Provider

Invitrogen Antibodies

Product name

SEC13 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji. Predicted reactivity: Mouse (97%), Rat (97%), Zebrafish (87%), Xenopus laevis (88%), Chicken (94%), Rhesus Monkey (98%), Chimpanzee (99%), Bovine (96%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SEC13 protein isoform 1 using 30 µg of A) 293T (B) A431 and C) H1299 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a SEC13 protein isoform 1 polyclonal antibody (Product # PA5-21339) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using SEC13 Polyclonal Antibody (Product # PA5-21339). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with SEC13 Polyclonal Antibody (Product # PA5-21339) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of SEC13 protein isoform 1 showing staining in the vesicles of HepG2 cells. HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a SEC13 protein isoform 1 polyclonal antibody (Product # PA5-21339) diluted at 1:500. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of SEC13 protein isoform 1 showing staining in the vesicles of HepG2 cells. HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a SEC13 protein isoform 1 polyclonal antibody (Product # PA5-21339) diluted at 1:500. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SEC13 Polyclonal Antibody detects SEC13L1 protein at COP II vesicles by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SEC13L1 protein stained by SEC13 Polyclonal Antibody (Product # PA5-21339) diluted at 1:500. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SEC13 Polyclonal Antibody detects SEC13L1 protein at COP II vesicles by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SEC13L1 protein stained by SEC13 Polyclonal Antibody (Product # PA5-21339) diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using SEC13L1 (Product # PA5-21339) antibody at 1:100 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SEC13L1 antibody immunoprecipitates SEC13L1 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 µg whole cell lysate/extract of SEC13L1 protein expressing HepG2 cells B : Control with 2.5 µg of pre-immune rabbit IgG C : Immunoprecipitation of SEC13L1 by 2.5 µg of SEC13L1 antibody (Product # PA5-21339) 10% SDS-PAGE The immunoprecipitated SEC13L1 protein was detected by SEC13L1 antibody (Product # PA5-21339) diluted at 1:1,000. Anti-rabbit IgG (HRP) was used as a secondary reagent.