PA5-21339

antibody from Invitrogen Antibodies

Targeting: SEC13 D3S1231E, npp-20, SEC13L1, SEC13R

Western blot

Immunoprecipitation

Immunohistochemistry

Antibody data

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Validation data

Product number: PA5-21339 - Provider product page
Provider: Invitrogen Antibodies
Product name: SEC13 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji. Predicted reactivity: Mouse (97%), Rat (97%), Zebrafish (87%), Xenopus laevis (88%), Chicken (94%), Rhesus Monkey (98%), Chimpanzee (99%), Bovine (96%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

SEC13 protein structure - PA5-21339 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of SEC13 protein isoform 1 showing staining in the vesicles of HepG2 cells. HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a SEC13 protein isoform 1 polyclonal antibody (Product # PA5-21339) diluted at 1:500. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of SEC13 protein isoform 1 showing staining in the vesicles of HepG2 cells. HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a SEC13 protein isoform 1 polyclonal antibody (Product # PA5-21339) diluted at 1:500. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: SEC13 Polyclonal Antibody detects SEC13L1 protein at COP II vesicles by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SEC13L1 protein stained by SEC13 Polyclonal Antibody (Product # PA5-21339) diluted at 1:500. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: SEC13 Polyclonal Antibody detects SEC13L1 protein at COP II vesicles by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SEC13L1 protein stained by SEC13 Polyclonal Antibody (Product # PA5-21339) diluted at 1:500. Blue: Hoechst 33342 staining.