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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-29261 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CARD10 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, H1299, Molt-4, Raji, mouse heart.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CARD10 using 30 µg of 293T lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a CARD10 polyclonal antibody (Product # PA5-29261) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CARD10 was performed by separating 50 µg of mouse tissue extract by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a CARD10 Polyclonal Antibody (Product # PA5-29261) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CARD10 Polyclonal Antibody detects CARD10 protein at cytoplasm on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. CARD10 Polyclonal Antibody (Product # PA5-29261) diluted at 1:250. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
