16024-1-AP

antibody from Proteintech Group

Targeting: LETM1 SLC55A1

Provider product page for 16024-1-AP

Western blot

ELISA

Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [20]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]
Immunohistochemistry [2]

Submit

Product number: 16024-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#16024-1-AP, RRID:AB_2136505
Product name: LETM1 antibody
Antibody type: Polyclonal
Description: KD/KO validated LETM1 antibody (Cat. #16024-1-AP) is a rabbit polyclonal antibody that shows reactivity with human and has been validated for the following applications: IF, IHC, WB,ELISA.
Reactivity: Human
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

LETM1 protein structure - 16024-1-AP shown in red.

Submitted references Nitric oxide-primed engineered extracellular vesicles restore bioenergetics in acute kidney injury via mitochondrial transfer.

Peng F, Chen X, Wu L, He J, Li Z, Hong Q, Zhao Q, Qian M, Wang X, Shen W, Qi T, Huang Y, Cai G, Zhang C, Chen X
Theranostics 2025;15(11):5499-5517

Integrative proximal-ubiquitomics profiling for deubiquitinase substrate discovery applied to USP30.

Damianou A, Jones HBL, Grigoriou A, Akbor MA, Jenkins E, Charles PD, Vendrell I, Davis S, Kessler BM
Cell chemical biology 2025 May 15;32(5):736-751.e8

Mitochondrial calcium homeostasis mediated by estradiol contributes to atrial fibrillation protection.

Lu B, Huang B, Wang Y, Ma G, Cai Z
Biochemical and biophysical research communications 2025 Jul 30;772:152050

Mdm38/LETM1 couples ion homeostasis and proteostatic mechanisms in the inner mitochondrial membrane.

Bohovych I, Menezes da Silva G, Ali SF, Bergmeyer EJ, Germany EM, Mayank A, Wohlschlegel JA, Casler JC, Rahman MA, Nazarko TY, Tarsio M, Shiota T, Lackner LL, Claypool SM, Kane PM, Barrientos A, Khalimonchuk O
bioRxiv : the preprint server for biology 2025 Jan 30;

Aberrant LETM1 elevation dysregulates mitochondrial functions and energy metabolism and promotes lung metastasis in osteosarcoma.

Shi Y, Kang Q, Zhou H, Yue X, Bi Y, Luo Q
Genes & diseases 2024 May;11(3):100988

Differential Effects of Three Medium-Chain Fatty Acids on Mitochondrial Quality Control and Skeletal Muscle Maturation.

Nishida R, Nukaga S, Kawahara I, Miyagawa Y, Goto K, Nakashima C, Luo Y, Sasaki T, Fujii K, Ohmori H, Ogata R, Mori S, Fujiwara-Tani R, Kuniyasu H
Antioxidants (Basel, Switzerland) 2024 Jul 9;13(7)

Caprylic Acid Inhibits High Mobility Group Box-1-Induced Mitochondrial Damage in Myocardial Tubes.

Nukaga S, Fujiwara-Tani R, Nishida R, Miyagawa Y, Goto K, Kawahara I, Nakashima C, Fujii K, Ogata R, Ohmori H, Kuniyasu H
International journal of molecular sciences 2024 Jul 24;25(15)

FAM210A is essential for cold-induced mitochondrial remodeling in brown adipocytes.

Qiu J, Yue F, Zhu P, Chen J, Xu F, Zhang L, Kim KH, Snyder MM, Luo N, Xu HW, Huang F, Tao WA, Kuang S
Nature communications 2023 Oct 10;14(1):6344

Multiomics Approach Reveals an Important Role of BNIP3 in Myocardial Remodeling and the Pathogenesis of Heart Failure with Reduced Ejection Fraction.

Chaanine AH, Higgins L, Lauterboeck L, Markowski T, Yang Q, Delafontaine P
Cells 2022 May 6;11(9)

Mutations in Hcfc1 and Ronin result in an inborn error of cobalamin metabolism and ribosomopathy.

Chern T, Achilleos A, Tong X, Hill MC, Saltzman AB, Reineke LC, Chaudhury A, Dasgupta SK, Redhead Y, Watkins D, Neilson JR, Thiagarajan P, Green JBA, Malovannaya A, Martin JF, Rosenblatt DS, Poché RA
Nature communications 2022 Jan 10;13(1):134

Sunitinib and Pterostilbene Combination Treatment Exerts Antitumor Effects in Gastric Cancer via Suppression of PDZD8.

Hojo Y, Kishi S, Mori S, Fujiwara-Tani R, Sasaki T, Fujii K, Nishiguchi Y, Nakashima C, Luo Y, Shinohara H, Kuniyasu H
International journal of molecular sciences 2022 Apr 4;23(7)

A common genetic variant of a mitochondrial RNA processing enzyme predisposes to insulin resistance.

Rossetti G, Ermer JA, Stentenbach M, Siira SJ, Richman TR, Milenkovic D, Perks KL, Hughes LA, Jamieson E, Xiafukaiti G, Ward NC, Takahashi S, Gray N, Viola HM, Hool LC, Rackham O, Filipovska A
Science advances 2021 Sep 24;7(39):eabi7514

Minor contribution of NCX to Na(+)-Ca(2+) exchange activity in brain mitochondria.

Takeuchi A, Matsuoka S
Cell calcium 2021 Jun;96:102386

HOXB7 acts as an oncogenic biomarker in head and neck squamous cell carcinoma.

Wu X, Li J, Yan T, Ke X, Li X, Zhu Y, Yang J, Li Z
Cancer cell international 2021 Jul 24;21(1):393

Total Matrix Ca(2+) Modulates Ca(2+) Efflux via the Ca(2+)/H(+) Exchanger in Cardiac Mitochondria.

Natarajan GK, Glait L, Mishra J, Stowe DF, Camara AKS, Kwok WM
Frontiers in physiology 2020;11:510600

Evaluation of cancer-derived myocardial impairments using a mouse model.

Miyagawa Y, Nukaga S, Mori T, Fujiwara-Tani R, Fujii K, Mori S, Goto K, Kishi S, Sasaki T, Nakashima C, Ohmori H, Kawahara I, Luo Y, Kuniyasu H
Oncotarget 2020 Oct 13;11(41):3712-3722

Intake of medium-chain fatty acids induces myocardial oxidative stress and atrophy.

Miyagawa Y, Mori T, Goto K, Kawahara I, Fujiwara-Tani R, Kishi S, Sasaki T, Fujii K, Ohmori H, Kuniyasu H
Lipids in health and disease 2018 Nov 17;17(1):258

The mitochondrial Na(+)/Ca(2+) exchanger is essential for Ca(2+) homeostasis and viability.

Luongo TS, Lambert JP, Gross P, Nwokedi M, Lombardi AA, Shanmughapriya S, Carpenter AC, Kolmetzky D, Gao E, van Berlo JH, Tsai EJ, Molkentin JD, Chen X, Madesh M, Houser SR, Elrod JW
Nature 2017 May 4;545(7652):93-97

The Mitochondrial Calcium Uniporter Matches Energetic Supply with Cardiac Workload during Stress and Modulates Permeability Transition.

Luongo TS, Lambert JP, Yuan A, Zhang X, Gross P, Song J, Shanmughapriya S, Gao E, Jain M, Houser SR, Koch WJ, Cheung JY, Madesh M, Elrod JW
Cell reports 2015 Jul 7;12(1):23-34

Letm1, the mitochondrial Ca2+/H+ antiporter, is essential for normal glucose metabolism and alters brain function in Wolf-Hirschhorn syndrome.

Jiang D, Zhao L, Clish CB, Clapham DE
Proceedings of the National Academy of Sciences of the United States of America 2013 Jun 11;110(24):E2249-54

No comments: Submit comment

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HEK-293 cells were subjected to SDS PAGE followed by western blot with 16024-1-AP(LETM1 antibody) at dilution of 1:500
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of Hela cells, using LETM1 antibody 16024-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human ovary tumor using 16024-1-AP(LETM1 antibody) at dilution of 1:100 (under 40x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human ovary tumor using 16024-1-AP(LETM1 antibody) at dilution of 1:100 (under 10x lens)
Sample type: tissue