Antibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00003954-M03 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00003954-M03, RRID:AB_540162
- Product name
- Mouse Monoclonal LETM1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. LETM1 - leucine zipper-EF-hand containing transmembrane protein 1
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Identification of LETM1 as a marker of cancer stem-like cells and predictor of poor prognosis in esophageal squamous cell carcinoma.
LETM1 overexpression is correlated with the clinical features and survival outcome of breast cancer.
High expression of leucine zipper-EF-hand containing transmembrane protein 1 predicts poor prognosis in head and neck squamous cell carcinoma.
LETM1-dependent mitochondrial Ca2+ flux modulates cellular bioenergetics and proliferation.
Co-delivery of LETM1 and CTMP synergistically inhibits tumor growth in H-ras12V liver cancer model mice.
Regulation of OPA1-mediated mitochondrial fusion by leucine zipper/EF-hand-containing transmembrane protein-1 plays a role in apoptosis.
Association of LETM1 and MRPL36 contributes to the regulation of mitochondrial ATP production and necrotic cell death.
LETM1, deleted in Wolf-Hirschhorn syndrome is required for normal mitochondrial morphology and cellular viability.
Yang Z, Ni W, Cui C, Qi W, Piao L, Xuan Y
Human pathology 2018 Nov;81:148-156
Human pathology 2018 Nov;81:148-156
LETM1 overexpression is correlated with the clinical features and survival outcome of breast cancer.
Li N, Zheng Y, Xuan C, Lin Z, Piao L, Liu S
International journal of clinical and experimental pathology 2015;8(10):12893-900
International journal of clinical and experimental pathology 2015;8(10):12893-900
High expression of leucine zipper-EF-hand containing transmembrane protein 1 predicts poor prognosis in head and neck squamous cell carcinoma.
Chen L, Yang Y, Liu S, Piao L, Zhang Y, Lin Z, Li Z
BioMed research international 2014;2014:850316
BioMed research international 2014;2014:850316
LETM1-dependent mitochondrial Ca2+ flux modulates cellular bioenergetics and proliferation.
Doonan PJ, Chandramoorthy HC, Hoffman NE, Zhang X, Cárdenas C, Shanmughapriya S, Rajan S, Vallem S, Chen X, Foskett JK, Cheung JY, Houser SR, Madesh M
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2014 Nov;28(11):4936-49
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2014 Nov;28(11):4936-49
Co-delivery of LETM1 and CTMP synergistically inhibits tumor growth in H-ras12V liver cancer model mice.
Shin JY, Chung YS, Kang B, Jiang HL, Yu DY, Han K, Chae C, Moon JH, Jang G, Cho MH
Cancer gene therapy 2013 Mar;20(3):186-94
Cancer gene therapy 2013 Mar;20(3):186-94
Regulation of OPA1-mediated mitochondrial fusion by leucine zipper/EF-hand-containing transmembrane protein-1 plays a role in apoptosis.
Piao L, Li Y, Kim SJ, Sohn KC, Yang KJ, Park KA, Byun HS, Won M, Hong J, Hur GM, Seok JH, Shong M, Sack R, Brazil DP, Hemmings BA, Park J
Cellular signalling 2009 May;21(5):767-77
Cellular signalling 2009 May;21(5):767-77
Association of LETM1 and MRPL36 contributes to the regulation of mitochondrial ATP production and necrotic cell death.
Piao L, Li Y, Kim SJ, Byun HS, Huang SM, Hwang SK, Yang KJ, Park KA, Won M, Hong J, Hur GM, Seok JH, Shong M, Cho MH, Brazil DP, Hemmings BA, Park J
Cancer research 2009 Apr 15;69(8):3397-404
Cancer research 2009 Apr 15;69(8):3397-404
LETM1, deleted in Wolf-Hirschhorn syndrome is required for normal mitochondrial morphology and cellular viability.
Dimmer KS, Navoni F, Casarin A, Trevisson E, Endele S, Winterpacht A, Salviati L, Scorrano L
Human molecular genetics 2008 Jan 15;17(2):201-14
Human molecular genetics 2008 Jan 15;17(2):201-14
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: LETM1 Antibody (6F7) [H00003954-M03] - Analysis of LETM1 expression in transfected 293T cell line by LETM1 monoclonal antibody (M03), clone 6F7. Lane 1: LETM1 transfected lysatE (83.4 KDa). Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: LETM1 Antibody (6F7) [H00003954-M03] - Detection limit for recombinant GST tagged LETM1 is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: LETM1 Antibody (6F7) [H00003954-M03] - Analysis of monoclonal antibody to LETM1 on HeLa cell. Antibody concentration 10 ug/ml
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: LETM1 Antibody (6F7) [H00003954-M03] - Analysis of LETM1 transfected lysate using anti-LETM1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with LETM1 rabbit polyclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: LETM1 Antibody (6F7) [H00003954-M03] - Analysis of monoclonal antibody to LETM1 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml